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Zoekresultaten - 11 results

Laboratory validation of two real-time RT-PCR methods with 5'-tailed primers for an enhanced detection of foot-and-mouth disease virus.

purposes, both assays were run in parallel in a triplex one-step RT-qPCR protocol with beta-actin as an internal control and synthetic RNA as an external control. We obtained low limits of detection and high ...

Influence of dabigatran and rivaroxaban on routine coagulation assays. A nationwide Belgian survey702

variability between reagents. Among aPTT reagents, Actin FSL (R) was the least sensitive to dabigatran while the other aPTT reagents showed slightly higher sensitivities. The presence of dabigatran led to ...

Influence of dabigatran and rivaroxaban on routine coagulation assays

® and Thromborel S® the least sensitive. Converting PT results to INR only increased the variability between reagents. Among aPTT reagents, Actin FSL ® was the least sensitive to dabigatran while the ...

Influence of dabigatran and rivaroxaban on routine coagulation assays. A nationwide Belgian survey647

variability between reagents. Among aPTT reagents, Actin FSL (R) was the least sensitive to dabigatran while the other aPTT reagents showed slightly higher sensitivities. The presence of dabigatran led to ...

The taxonomic status of Trichophyton quinckeanum and T. interdigitale revisited: a multigene phylogenetic approach.

p.871-82 (2012) Keywords: Actins Animals Cluster Analysis DNA, Fungal DNA, Ribosomal Spacer Genes, Fungal Humans mice Molecular Sequence Data Phylogeny Sequence Analysis, DNA Trichophyton Tubulin Abstract: ...

Development and inter-laboratory validation study of an improved new real-time PCR assay with internal control for detection and laboratory diagnosis of African swine fever virus.

Marie-Frédérique; Bishop, Richard P; Arias, Marisa; F. Koenen Source: J Virol Methods, Volume 178, Issue 1-2, p.161-70 (2011) Keywords: Actins African Swine Fever African Swine Fever Virus Animals Capsid Proteins ...

Effect of pooling and multiplexing on the detection of bluetongue virus RNA by real-time RT-PCR.

152, Issue 1-2, p.13-7 (2008) Keywords: Actins Animals Bluetongue Bluetongue virus Cattle Cattle Diseases Reverse Transcriptase Polymerase Chain Reaction RNA, Viral Sheep Abstract: Real-time RT- PCR ...

A universal avian endogenous real-time reverse transcriptase-polymerase chain reaction control and its application to avian influenza diagnosis and quantification.

a universal avian endogenous internal control (bird beta-actin) and apply it to influenza A diagnosis. Moreover, this internal control proves useful as a normalizer control for virus quantification, because ...

Bluetongue virus detection by two real-time RT-qPCRs targeting two different genomic segments.

strains. Therefore these two methods are complementary and could be used in parallel to confirm the diagnosis of a possible new introduction of BTV. An RT-qPCR amplifying a fragment of the beta-actin mRNA ...

Binding and entry characteristics of porcine circovirus 2 in cells of the porcine monocytic line 3D4/31.

s co-localized with clathrin. PCV2 infection was decreased significantly by chemical inhibitors that specifically blocked (i) actin-dependent processes, including cytochalasin D (75.5+/-7.0 % reduction) and ...

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