Zoekresultaten - 9 results
A Tale of Tails: Dissecting the Enhancing Effect of Tailed Primers in Real-Time PCR.
Sequence analysis of the target region of these 5 isolates revealed several mutations in the inter-primer region that extend an existing hairpin structure immediately downstream of the forward primer binding ...
The RNA template channel of the RNA-dependent RNA polymerase as a target for development of antiviral therapy of multiple genera within a virus family.
Cameron, Craig E; Verdaguer, Nuria; Neyts, Johan; van Kuppeveld, Frank J M Source: PLoS Pathog, Volume 11, Issue 3, p.e1004733 (2015) Keywords: Animals Antiviral Agents Binding Sites Cardiovirus Chlorocebus ...
Application of a cell-based protease assay for testing inhibitors of picornavirus 3C proteases.
proteases. The assay is based on the induction of expression of a firefly luciferase reporter by a chimeric transcription factor in which the viral protease and cleavage sites are inserted between the GAL4 ...
Antibody evasion by a gammaherpesvirus O-glycan shield.
involved in cell binding, and some provide neutralization targets. However, the capacity of gammaherpesviruses for long-term transmission from immune hosts implies that in vivo neutralization is incomplete. ...
Identification of a dominant epitope in the hemagglutinin of an Asian highly pathogenic avian influenza H5N1 clade 1 virus by selection of escape mutants.
viruses had Lys (K) at position 189 in the HA1, whereas HA sequences analyzed from dade 2.1 and 2.2 viruses had Arg (R). This residue also interacts with the receptor-binding site, and it is thus important ...
A pyrazolotriazolopyrimidinamine inhibitor of bovine viral diarrhea virus replication that targets the viral RNA-dependent RNA polymerase.
binding site as BPIP or VP32947 at the top of the finger domain of the polymerase that is a “hot spot” for inhibition of pestivirus replication. Health Topics: Animal health Category: A1 FOS ...
The imidazopyrrolopyridine analogue AG110 is a novel, highly selective inhibitor of pestiviruses that targets the viral RNA-dependent RNA polymerase at a hot spot for inhibition of viral replication.
J Virol, Volume 81, Issue 20, p.11046-53 (2007) Keywords: Animals Antiviral Agents Binding Sites Cattle Diarrhea Viruses, Bovine Viral Enzyme Inhibitors Models, Molecular Pestivirus Pestivirus Infections ...
Identification of DNA sequences recognized by VirF, the transcriptional activator of the Yersinia yop regulon.
vitro and that specifically binds to the promoter region of VirF-regulated genes. In this work, we determined the sequences of eight VirF-binding sites from four different genes, by DN ase I or hydroxyl ...
YscN, the putative energizer of the Yersinia Yop secretion machinery.
Triphosphatases Amino Acid Sequence Bacterial Outer Membrane Proteins Bacterial Proteins Base Sequence Carrier Proteins Cloning, Molecular Genes, Bacterial Molecular Sequence Data Mutagenesis, Site ...