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Zoekresultaten - 75 results
Classical swine fever virus detection: results of a real-time reverse transcription polymerase chain reaction ring trial conducted in the framework of the European network of excellence for epizootic disease diagnosis and control.
the acceptable range. The FLI assay, several in-house assays, and the commercial kits had high analytical sensitivity and specificity values. Nevertheless, some in-house systems had unspecific reactions ...
Diagnostic performance and application of two commercial cell viability assays in foot-and-mouth disease research.
commercial cell viability assays (CellTiter 96(®) AQ ueous One Solution Cell Proliferation Assay (MTS) and CellTiter-Blue(®) Cell Viability Assay (CTB), both from Promega, Leiden, The Netherlands) was ...
Persistence of free-living protozoan communities across rearing cycles in commercial poultry houses.
strategies. We therefore investigated the spatial and temporal dynamics of free-living protozoa in three habitats (pipeline, water, and miscellaneous samples) in three commercial poultry houses across three ...
Cross-reactivity of antibodies in some commercial deoxynivalenol test kits against some fusariotoxins.
Publication Type: Peer reviewed scientific article Authors: Emmanuel Tangni; Motte, Jean-Claude; A. Callebaut; Pussemier, Luc Source: J Agric Food Chem, Volume 58, Issue 24, p.12625-33 (2010) Keywords: Antibodies, Fungal Cross Reactions Enzyme-Linked Immu ...
A non-invasive fluorescent staining procedure allows Confocal Laser Scanning Microscopy based imaging of Mycobacterium in multispecies biofilms colonizing and degrading polycyclic aromatic hydrocarbons.
signal produced by commonly used fluorescent reporter genes. The procedure makes use of the commercial non-specific nucleic acid stain Syto62 and was optimized to efficiently stain Mycobacterium cells in ...
Passive protection afforded by maternally-derived antibodies in chickens and the antibodies' interference with the protection elicited by avian influenza-inactivated vaccines in progeny.
plan that could be applied nationwide to all commercial and backyard poultry. However, after several months of vaccination in enzootic areas, maternally derived antibody (MDA) is present in young chicks, ...
Evaluation of rapid antigen detection kits for the diagnosis of highly pathogenic avian influenza H5N1 infection.
commercially available, but only a few of them have been evaluated. In this study, four commercially available rapid tests for veterinary usage and two tests for human usage were evaluated and compared. The ...
Improved vaccination against Newcastle disease by an in ovo recombinant HVT-ND combined with an adjuvanted live vaccine at day-old.
fatal Newcastle disease (ND) in commercial poultry flocks demonstrate that current vaccination strategies are not fully efficacious and should be improved by new generation of vaccines. In this context, ...
Emergence of bluetongue serotypes in Europe, part 1: description and validation of four real-time RT-PCR assays for the serotyping of bluetongue viruses BTV-1, BTV-6, BTV-8 and BTV-11.
compared with commercially available kits for the detection of BTV-1, BTV-6 and BTV-8. Three in-house assays gave exactly the same diagnostic result (positive/negative) as the commercial assays and can thus ...
Validation of two real-time RT-PCR methods for foot-and-mouth disease diagnosis: RNA-extraction, matrix effect, uncertainty of measurement and precision.
not performed or reported generally. In this study, different RNA-extraction procedures were compared for two FMDV rRT- PCR s. The NucleoSpin columns available commercially combined high extraction ...