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Deepening of In Silico Evaluation of SARS-CoV-2 Detection RT-qPCR Assays in the Context of New Variants

Source: Genes, Volume 12, Issue 4 (2021) Keywords: SARS-CoV-2; COVID-19; variants; detection; RT-qPCR; in silico specificity evaluation; bioinformatics tool; WGS data; mismatches; primers and probes ...

Use of Whole Genome Sequencing Data for a First in Silico Specificity Evaluation of the RT-qPCR Assays Used for SARS-CoV-2 Detection.

a total of 30 primers and probe sets) used for SARS-CoV-2 detection and the impact of the virus’ genetic evolution on four of them. The exclusivity of these methods was also assessed using the human ...

Piacentinu Ennese PDO cheese as reservoir of promising probiotic bacteria.

genomic fingerprinting, using the (GTG) 5-primer, and by MALDI- TOF MS. One hundred and thirteen (113) isolates belonging to QPS-list species were characterized for both safety and functional properties. ...

Detection and discrimination of five E. coli pathotypes using a combinatory SYBR® Green qPCR screening system

genes. uidA controls for E. coli presence and all the other genes are specific targets of E. coli pathotypes. For each gene, two primer pairs have been designed to guarantee a sufficient detection even in ...

Laboratory validation of two real-time RT-PCR methods with 5'-tailed primers for an enhanced detection of foot-and-mouth disease virus.

Keywords: 5' Untranslated Regions Animals DNA Primers Foot-and-Mouth Disease Foot-and-Mouth Disease Virus Reproducibility of Results Reverse Transcriptase Polymerase Chain Reaction RNA, Viral Sensitivity ...

A Versatile Panel of Reference Gene Assays for the Measurement of Chicken mRNA by Quantitative PCR.

efficiency, and not produce signals from contaminating DNA. Whilst recent research papers identify specific genes that are stable in particular tissues and experimental treatments, here we describe a panel of ...

A Tale of Tails: Dissecting the Enhancing Effect of Tailed Primers in Real-Time PCR.

p.e0164463 (2016) Keywords: Animals DNA Primers Foot-and-Mouth Disease Virus Humans Real-Time Polymerase Chain Reaction Abstract: Non-specific tail sequences are often added to the 5’-terminus of primers to ...

Development and performance assessment of a qualitative SYBR® green real-time PCR assay for the detection of Aspergillus versicolor in indoor air.

versicolor, which is frequently observed in indoor environment and known to be allergenic. The developed primers amplify a short region of the internal transcribed spacer 1 from the 18S ribosomal DNA complex. ...

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