Zoekresultaten - 11 results
Adjunct Culture of Non-Starter Lactic Acid Bacteria for the Production of Provola Dei Nebrodi PDO Cheese: In Vitro Screening and Pilot-Scale Cheese-Making.
were genetically identified by Rep- PCR genomic fingerprinting, using the (GTG)-primer, and by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI- TOF MS). Twenty-seven ...
Adjunct Culture of Non-Starter Lactic Acid Bacteria for the Production of Provola Dei Nebrodi PDO Cheese: In Vitro Screening and Pilot-Scale Cheese-Making
(GTG)5-primer, and by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI- TOF MS). Twenty-seven strains, included in the qualified presumption of safety (QPS) list, were tested for ...
Piacentinu Ennese PDO cheese as reservoir of promising probiotic bacteria.
genomic fingerprinting, using the (GTG) 5-primer, and by MALDI- TOF MS. One hundred and thirteen (113) isolates belonging to QPS-list species were characterized for both safety and functional properties. ...
Development and performance assessment of a qualitative SYBR® green real-time PCR assay for the detection of Aspergillus versicolor in indoor air.
versicolor, which is frequently observed in indoor environment and known to be allergenic. The developed primers amplify a short region of the internal transcribed spacer 1 from the 18S ribosomal DNA complex. ...
Guidelines for optimisation of a multiplex oligonucleotide ligation-PCR for characterisation of microbial pathogens in a microsphere suspension array.
p.790170 (2015) Keywords: DNA Primers DNA, Bacterial Humans Microspheres Multiplex Polymerase Chain Reaction Oligonucleotide Probes Salmonella typhimurium Abstract: With multiplex oligonucleotide ligation- ...
A two-step lyssavirus real-time polymerase chain reaction using degenerate primers with superior sensitivity to the fluorescent antigen test.
two-step lyssavirus real-time reverse transcriptase polymerase chain reaction (qRT- PCR), based on a nested PCR strategy, was validated for the detection of different lyssavirus species. Primers with 17 to ...
SYBR®Green qPCR Salmonella detection system allowing discrimination at the genus, species and subspecies levels.
Issue 22, p.9811-24 (2013) Keywords: Bacterial Proteins Bacteriological Techniques DNA Primers Fluorescent Dyes Organic Chemicals Real-Time Polymerase Chain Reaction Reproducibility of Results Salmonella ...
An innovative and integrated approach based on DNA walking to identify unauthorised GMOs
cassette and the rice genome. To this end, a DNA walking strategy is applied using a first reverse primer followed by two semi-nested PCR rounds using primers that are each time nested to the previous ...
An innovative integrated approach based on DNA walking to identify unauthorized GMOs
of the junction between the transgenic cassette and the rice genome. To this end, a DNA walking strategy is applied using a first reverse primer followed by two semi-nested PCR rounds using primers ...
Development and validation of qualitative SYBR®Green real-time PCR for detection and discrimination of Listeria spp. and Listeria monocytogenes.
of Listeria spp. The selected primers were designed to amplify short fragments (60 to 103 bp) in order to obtain optimal PCR efficiency (between 97 and 107 % efficiency). The limit of detection of the ...