Zoekresultaten - 6 results

Towards Real-Time and Affordable Strain-Level Metagenomics-Based Foodborne Outbreak Investigations Using Oxford Nanopore Sequencing Technologies

Flongle flow cell, on an extract treated with eukaryotic host DNA removal. Moreover, an alternative approach based on in silico  DNA walking allowed to obtain rapid confirmation of the presence of ...

Whole Genome Sequencing Provides an Added Value to the Investigation of Staphylococcal Food Poisoning Outbreaks

C.J. De Keersmaecker Source: Frontiers in Microbiology, Volume 12 (2021) Keywords: DNA extraction kits enterotoxin gene profiling outbreak investigation relatedness determination Staphylococcus aureus, ...

A Practical Method to Implement Strain-Level Metagenomics-Based Foodborne Outbreak Investigation and Source Tracking in Routine.

Shiga toxin-producing (STEC) as a case study. Different DNA extraction kits and enrichment procedures were investigated to obtain the most practical workflow. We demonstrated the feasibility of shotgun ...

Toxoplasma gondii in stranded marine mammals from the North Sea and Eastern Atlantic Ocean: Findings and diagnostic difficulties

Protozoan Aquatic Organisms Atlantic Ocean Cetacea Diagnostic Tests, Routine DNA, Protozoan ELISA Enzyme-Linked Immunosorbent Assay Fluorescent Antibody Technique IFA Mammals Marine mammals MAT North Sea ...

Toxoplasma gondii and Neospora caninum in wildlife: common parasites in Belgian foxes and Cervidae?

Source: Vet Parasitol, Volume 178, Issue 1-2, Number 69, p.64-9 (2011) Keywords: Animals Belgium brain Coccidiosis Deer DNA, Protozoan Foxes Genotype Neospora Toxoplasma Toxoplasmosis, Animal Abstract: Sera ...

Impact of DNA extraction on whole genome sequencing analysis for characterization and relatedness of Shiga toxin-producing Escherichia coli isolates.

characterization and relatedness determination. However, standardized and harmonized workflows, e.g. for DNA extraction, are required to ensure robust and exchangeable WGS data. Data sharing between (inter)national ...

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