Zoekresultaten - 24 results

Development and performance assessment of a qualitative SYBR® green real-time PCR assay for the detection of Aspergillus versicolor in indoor air.

Subsequently, the performance of this quantitative polymerase chain reaction (qPCR) method was assessed using specific criteria, including an evaluation of the selectivity, PCR efficiency, dynamic range, and ...

Exposure determinants of cadmium in European mothers and their children.

European countries. The aim of the study was to evaluate the overall Cd exposure and significant determinants of Cd exposure. A study population of 1632 women (24-52 years of age), and 1689 children (5-12 ...

Guidelines for optimisation of a multiplex oligonucleotide ligation-PCR for characterisation of microbial pathogens in a microsphere suspension array.

targeted bacterial species, evaluation of the method of DNA isolation, the probe concentration, the amount of microspheres, and the concentration of reporter dye is advisable in the development of any MOL- ...

Evaluation of viability-qPCR detection system on viable and dead Salmonella serovar Enteritidis.

Enteritidis. The PMA treatment protocol was modified (dark incubation duration, concentration of PMA) to evaluate if a complete negative signal of dead Salmonella was possible. However, none of these ...

Foodborne cereulide causes beta-cell dysfunction and apoptosis.

toxicity were evaluated in MIN6 cells by mRNA profiling, electron microscopy and mitochondrial function tests. RESULTS: 24 h exposure to 5 ng/ml cereulide rendered almost all MIN6, INS-1E and pancreatic ...

2,4-Dialkyl-8,9,10,11-tetrahydrobenzo[g]pyrimido[4,5-c]isoquinoline-1,3,7,12(2H,4H)-tetraones as new leads against Mycobacterium tuberculosis.

dialkyltetrahydrobenzo[g]pyrimido [4,5-c]isoquinolinetetraones were designed and synthesised. The compounds were submitted to a biological evaluation in which the activity against M.tb H37R v(lux) was observed, as well as the acute ...

Dietary intake of lycopene by the Belgian adult population.

lycopene is associated with a decreased risk of various chronic diseases. The aim of the present study was to evaluate the distribution of dietary lycopene intake in the Belgian population and to determine ...

In vitro model to study the endocrine disrupting activity of migration products from plastic food contact materials

polyamide, polystyrene, and melamine. The second part of this work will be to evaluate the endocrine disruptor activity(ies) of global migration residues obtained from different kinds of baby bottles. This ...

SYBR®Green qPCR Salmonella detection system allowing discrimination at the genus, species and subspecies levels.

coding for a cytoplasmic protein. This study includes the design of primer pairs, in silico and in situ selectivity, sensitivity, repeatability and reproducibility evaluations of the seven SYBR ®Green qPCR ...

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