Zoekresultaten - 6 results

Detection and discrimination of five E. coli pathotypes using a combinatory SYBR® Green qPCR screening system

silico and in situ selectivity, sensitivity, repeatability, and reproducibility evaluation of the 13 SYBR ® Green qPCR assays. Each target displayed a selectivity of 100%. The limit of detection of the 13 ...

Growth of Stressed Strains of Four Non-O157 Shiga Toxin-Producing Escherichia coli Serogroups in Five Enrichment Broths.

Keywords: Culture Media Serogroup Shiga-Toxigenic Escherichia coli Abstract: The purpose of this study was to evaluate (i) the behavior of several strains of non- O157 Shiga toxin-producing Escherichia coli ...

Comparison of Six Chromogenic Agar Media for the Isolation of a Broad Variety of Non-O157 Shigatoxin-Producing Escherichia coli (STEC) Serogroups.

selection of a suitable selective isolation agar remains problematic. The purpose of this study was to qualitatively and quantitatively evaluate six chromogenic agar media for the isolation of STEC: Tryptone ...

Comparison of Droplet Digital PCR and qPCR for the Quantification of Shiga Toxin-Producing Escherichia coli in Bovine Feces.

UMM: TaqMan(®) Universal PCR Master Mix) were evaluated, using standard curves and both artificial and natural contaminated cattle fecal samples. In addition, the susceptibility of these assays to ...

Impact of DNA extraction on whole genome sequencing analysis for characterization and relatedness of Shiga toxin-producing Escherichia coli isolates.

laboratories is essential to support foodborne pathogen control, including outbreak investigation. This study evaluated eight commercial DNA preparation kits for their potential influence on: (i) DNA quality for ...

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