Zoekresultaten - 13 results

Targeted High-Throughput Sequencing Enables the Detection of Single Nucleotide Variations in CRISPR/Cas9 Gene-Edited Organisms

sequencing PCR-based enrichment single nucleotide variations Transgenic Rice Abstract: Similar to genetically modified organisms (GMO s) produced by classical genetic engineering, gene-edited (GE) organisms ...

Development of a taxon-specific real-time PCR method targeting the Bacillus subtilis group to strengthen the control of genetically modified bacteria in fermentation products

complying with the Minimum Performance Requirements for Analytical Methods of GMO Testing that is used as a standard by the GMO enforcement laboratories. The method’s applicability was also tested on 25 ...

Detection and identification of authorized and unauthorized GMOs using high-throughput sequencing with the support of a sequence-based GMO database

Publication Type: Peer reviewed scientific article Authors: Assia Saltykova; Julien Van Braekel; N. Papazova; Marie-Alice Fraiture; Deforce, Dieter; Kevin Vanneste; Sigrid C.J. De Keersmaecker; Nancy Roosens Source: Food Chemistry: Molecular Sciences, Vol ...

Development of a real-time PCR marker targeting a new unauthorized genetically modified microorganism producing protease identified by DNA walking

real-time PCR GMM protease2 marker was also demonstrated on microbial fermentation products. To confirm its use by other GMO enforcement laboratories, the transferability of the in-house validated real-time ...

How can we better detect unauthorized GMO in the food and feed chain

6, Number 517 (2017) Keywords: detection DNA walking Monitoring new workflow next-generation-sequencing qPCR unauthorized GMO Abstract: Current GMO detection systems have limited abilities to detect ...

An integrated strategy combining DNA walking and NGS to detect GMO

Volume 232, Number 358 (2017) Keywords: detection DNA walking GMO Next-generation sequencing qPCR Abstract: Recently, we developed a DNA walking system for the detection and characterization of a broad ...

Biotech Rice: Current developments and future detection challenges in food and feed chain

were developed, including transgenesis commonly using Agrobacterium tumefaciens or biolistic technologies. To guarantee the traceability of GMO in food/feed chain and the consumer's freedom of ...

Current and new approaches in GMO detection: challenges and solutions

International, Volume 2015, Issue 392872 (2015) Keywords: Current and New Approaches in GMO Detection: Challenges and Solutions Abstract: In many countries, genetically modified organisms (GMO) legislations have ...

Integrated DNA walking system to characterize a broad spectrum of GMOs in food/feed matrices.

with qPCR screening, usually used in GMO routine analysis, as well as being able to detect, characterize and identify a broad spectrum of GMO s in food/feed matrices, two bidirectional DNA walking ...

Statistical framework for detection of Genetically Modified Organisms based on Next Generation Sequencing

Nieuwerburgh, Filip; Nancy Roosens Source: Food Chemistry, Volume 192, Issue 1, Number 798 (2016) Keywords: bioinformatics detection GM rice GMO NGS Processed food Statistical framework Abstract: Because the ...

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