Zoekresultaten - 627 results

Perceived utility and feasibility of pathogen genomics for public health practice: a survey among public health professionals working in the field of infectious diseases, Belgium, 2019

Publication Type: Peer reviewed scientific article Authors: Nina Van Goethem; M. J. Struelens; Sigrid C.J. De Keersmaecker; Nancy Roosens; A. Robert; Sophie Quoilin; Herman Van Oyen; Brecht Devleesschauwer Source: BMC Public Health, Volume 20, Issue 1 (20 ...

Impact of DNA extraction on whole genome sequencing analysis for characterization and relatedness of Shiga toxin-producing Escherichia coli isolates

C.J. De Keersmaecker Source: Scientific Reports, Volume 10, Issue 1 (2020) Keywords: DNA extraction kits outbreak investigation Shiga toxin producing Escherichia coli (STEC) Whole genome sequencing (WGS) ...

Combining short and long read sequencing to characterize antimicrobial resistance genes on plasmids applied to an unauthorized genetically modified Bacillus

a major public health threat. Plasmids are able to transfer AMR genes among bacterial isolates. Whole genome sequencing (WGS) is a powerful tool to monitor AMR determinants. However, plasmids are difficult ...

Increased viral read counts and metagenomic full genome characterization of porcine astrovirus 4 and Posavirus 1 in sows in a swine farm with unexplained neonatal piglet diarrhea

Publication Type: Peer reviewed scientific article Authors: Steven Van Borm; Kevin Vanneste; Fu, Qiang; Maes, Dominiek; Alexandra Schoos; Eline Vallaey; Frank Vandenbussche Source: Virus Genes, Volume 56, Issue 6 (2020) Health Topics:  Animal health Santé ...

First detection of a plasmid located carbapenem resistant blaVIM-1 gene in E. coli isolated from meat products at retail in Belgium in 2015

Activités transversales en génomique appliquée Transversale activiteiten in toegepaste genomica Transversal activities in applied genomics Pathogènes alimentaires Voedselpathogenen Foodborne pathogens ...

A multiplex oligonucleotide ligation-PCR method for the genoserotyping of common Salmonella using a liquid bead suspension assay

appliquée Transversale activiteiten in toegepaste genomica Transversal activities in applied genomics Manuscript versions:  DOI:  https://doi.org/10.1016/j.fm.2019.103394 File:  Version:  Published Full text ...

Selection of a Noninvasive Source of Human DNA Envisaging Genotyping Assays in Epidemiological Studies: Urine or Saliva?

Genomic DNA was extracted using commercial kits. Quantitative and qualitative evaluation was done by assessing the yield, the purity, and integrity of the extracted DNA. As a proof-of-concept, genotyping ...

Next-Generation Sequencing: An Eye-Opener for the Surveillance of Antiviral Resistance in Influenza

antivirals can lead to antiviral resistance mutations in the entire influenza genome. Therefore, the methods to detect these mutations should be developed and implemented. In this Opinion, we assess how NGS ...

A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses

and chikungunya (CHIKV) viruses simultaneously. Methods Two methods targeting different genome segments were selected from the literature for each virus. These were adapted for high genome coverage and ...

Whole-genome sequencing of serotype 4b isolated from ready-to-eat lentil salad in Algiers, Algeria.

Microbes New Infect, Volume 33 (2020) Abstract: is a Gram-positive food-borne pathogen causing a serious threat for public health. Here we announce the whole genome sequence (3 011 693 bp) of serotype 4b, ...

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