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Zoekresultaten - 8 results
Development of a genoserotyping system for the identification of Salmonella serotypes
detection of molecular markers or on the analysis of sequencing data, are described in the scientific literature. Based on a critical review of some of these techniques, the Multiplex Oligonucleotide ...
Molecular Subtyping of Salmonella Typhimurium with Multiplex Oligonucleotide Ligation-PCR (MOL-PCR).
Biol, Volume 1616, p.39-69 (2017) Abstract: A multiplex oligonucleotide ligation- PCR (MOL- PCR) assay is a valuable high-throughput technique for the detection of bacteria and viruses, for ...
Development of a Luminex xTAG® assay for cost-effective multiplex detection of β-lactamases in Gram-negative bacteria.
identification of ESBL s and carbapenemases in Gram-negative bacilli. This assay had to be (i) superior to traditional (multiplex) PCR /sequencing-based tests in turn-around time, gene coverage and the ability to ...
A multiplex oligonucleotide ligation-PCR as a complementary tool for subtyping of Salmonella Typhimurium
foodborne infections. In an attempt to overcome the intrinsic shortcomings of currently used subtyping techniques, a multiplex oligonucleotide ligation- PCR (MOL- PCR) assay was developed which combines ...
Guidelines for optimisation of a multiplex oligonucleotide ligation-PCR for characterisation of microbial pathogens in a microsphere suspension array.
PCR (MOL- PCR) different molecular markers can be simultaneously analysed in a single assay and high levels of multiplexing can be achieved in high-throughput format. As such, MOL- PCR is a convenient ...
Molecular subtyping of Salmonella Typhimurium combining different types of markers in a multiplex liquid bead suspension array
Design detection disease Diseases Foodborne Diseases gene Genes Genome identification identify International IS Luminex method molecular subtyping Multiplex assay ON outbreak Phage type profile resistance ...
Serological profiles in nursery piglets colonized with Staphylococcus aureus.
Twenty-four out of the 31 piglets were challenged with MRSA ST398. The serum samples were analyzed for IgG antibodies to 39 S. aureus antigens, using a multiplex bead-based assay (xMAP technology, Luminex ...
Design of multiplex assays for molecular subtyping of pathogens with the Luminex xMAP technology
IT limitation Limitations Luminex microorganism microorganisms Molecular molecular subtyping Multiplex assay Oligonucleotides outbreak outbreaks pathogen reducing regulation Sample SOFTWARE specificity ...