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Zoekresultaten - 10 results
Strategies to detect unauthorized genetically modified organisms in food and feed chain
workflow and increase the throughput of this strategy, the sequencing step was performed using the Next Generation Sequencing (NGS) technology instead of the Sanger technology. In parallel, the detection of ...
The origin of biased sequence depth in sequence-independent nucleic acid amplification and optimization for efficient massive parallel sequencing.
a 3’ random part used to prime complementary DNA synthesis and a 5’ defined tag sequence for subsequent amplification. Recently, this amplification method was combined with next generation sequencing to ...
What's in a strain? Viral metagenomics identifies genetic variation and contaminating circoviruses in laboratory isolates of pigeon paramyxovirus type 1.
used next generation sequencing on random amplified viral nucleic acids to determine the genome sequence of 11 pigeon paramyxovirus type 1 (PPMV-1) isolates from Belgium (period 1998-2011). The PPMV-1 ...
Phylogeographic analysis of avian influenza viruses isolated from Charadriiformes in Belgium confirms intercontinental reassortment in gulls.
random amplification and next-generation sequencing. The gene segments of these viruses segregated into three phylogeographic lineage types: (1) segments circulating in waterfowl in Eurasia with sporadic ...
DNase SISPA-next generation sequencing confirms Schmallenberg virus in Belgian field samples and identifies genetic variation in Europe.
protocols for this novel virus we confirmed its presence in RT-qPCR positive field samples using DN ase SISPA-next generation sequencing (NGS), a virus discovery method based on random amplification and next ...
Identification and complete genome sequencing of paramyxoviruses in mallard ducks (Anas platyrhynchos) using random access amplification and next generation sequencing technologies.
next generation sequencing for the sequence independent identification of the viruses and the determination of their genomes. RESULTS: Three different APMV s were identified. From one pooled sample, the ...
Whole-genome-based phylogenomic analysis of the Belgian 2016-2017 influenza A(H3N2) outbreak season allows improved surveillance.
Organization (WHO) and European Centre for Disease Prevention and Control (ECDC) guidelines is based on combining certain genotypic amino acid mutations and phylogenetic analysis. Next-generation sequencing ...
Dietary silver nanoparticles can disturb the gut microbiota in mice.
Body weight, systemic inflammation and gut integrity were investigated to determine overall toxicity, and feces DNA collected from the gut were analyzed by Next Generation Sequencing (NGS) to assess the ...
Replicative fitness recuperation of a recombinant murine norovirus- reciprocity of genetic shift and drift.
were characterized via next-generation sequencing and putatively associated to fitness changes. To investigate the effect of genomic changes separately and in combination in the context of ...
Identification of an unauthorized genetically modified bacteria in food enzyme through whole-genome sequencing.
at the European level (RASFF 2019.3332). This finding was made possible thanks to the use of the next-generation sequencing technology, as reported in this study. Whole-genome sequencing was used to ...