Zoekresultaten - 9 results

Introduction of WGS in food microbiology: advantages and challenges

for the Safety of the Food Chain (FASFC) published the opinion 18-2021 regarding whole genome sequencing (WGS) for the detection of foodborne outbreaks and bacterial risk assessment. This opinion was ...

Towards Real-Time and Affordable Strain-Level Metagenomics-Based Foodborne Outbreak Investigations Using Oxford Nanopore Sequencing Technologies

Technologies, and we compared this to Illumina short-read-based metagenomics. After 12 h of MinION sequencing, strain-level characterization could be achieved, linking the food containing a pathogen to the ...

Whole Genome Sequencing Provides an Added Value to the Investigation of Staphylococcal Food Poisoning Outbreaks

of S. aureus isolates, including some belonging to a confirmed foodborne outbreak, its added value as the ultimate multiplexing method was demonstrated. In contrast to PCR-based se gene detection for ...

Staphylococcal enterotoxin gene cluster: prediction of enterotoxin (SEG and SEI) production and of the source of food poisoning based on Saβ typing.

derived). The amount of SEG and SEI produced also correlated with the Saβ type and the CC of a strain. The present results show promising indications that the production of SEG and SEI can be predicted based ...

The Benefits of Whole Genome Sequencing for Foodborne Outbreak Investigation from the Perspective of a National Reference Laboratory in a Smaller Country.

genotypes. Besides a universal, all-in-one workflow with less hands-on-time (five versus seven actual working days for WGS versus conventional), WGS-based cgMLST-typing demonstrated increased resolution. This ...

NAuRA: Genomic Tool to Identify Staphylococcal Enterotoxins in Strains Responsible for FoodBorne Outbreaks.

Hristo Daskalov; Michel-Yves Mistou; Jacques-Antoine Hennekinne Source: Front Microbiol, Volume 11 (2020) Keywords: bioinformatics tool; gene detection genomic staphylococcal enterotoxins genes variant ...

Detection and discrimination of five E. coli pathotypes using a combinatory SYBR® Green qPCR screening system

Microbiology and Biotechnology, Volume 102, Issue 7, Number 3285 (2018) Keywords: Detection; Pathogenic E. coli; Real-time PCR; STEC; SYBR® Green; Validation Abstract: A detection and discrimination system for ...

A pan-European ring trial to validate an International Standard for detection of Vibrio cholerae, Vibrio parahaemolyticus and Vibrio vulnificus in seafoods

molecular based methods for identification of human pathogenic Vibrio spp. classical and real-time PCR options were also included within the scope of the validation. These considerations formed the basis of ...

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