Zoekresultaten - 388 results

Detection of Clinical and Subclinical Lumpy Skin Disease Using Ear Notch Testing and Skin Biopsies.

Microorganisms, Volume 9, Issue 10 (2021) Abstract: Lumpy skin disease (LSD) diagnosis is primarily based on clinical surveillance complemented by PCR of lesion crusts or nodule biopsies. Since LSD can be ...

Development of a real-time PCR marker targeting a new unauthorized genetically modified microorganism producing protease identified by DNA walking

Microbiology, Volume 354 (2021) Keywords: Antimicrobial resistance genes DNA walking anchored on pUB110 food and feed safety Genetically modified bacteria Protease real-time PCR detection Abstract: A PCR-based ...

Development of a multiplex immunoassay for simultaneous detection of antigens in DTaP vaccines

that could be implemented for routine potency assessment of vaccines. Our work focused on the development of a Luminex-based multiplex assay (four to six-plex assay depending on the number of pertussis ...

Validation of TaqMan-Based Assays for Specific Detection and Differentiation of Wild-Type and Neethling Vaccine Strains of LSDV.

Petrović; Ilse De Leeuw; Andy Haegeman Source: Microorganisms, Volume 9, Issue 6 (2021) Keywords: differentiation DIVA diagnostic protocol field strain Lumpy skin disease virus Real-time PCR vaccine strain. ...

Towards Real-Time and Affordable Strain-Level Metagenomics-Based Foodborne Outbreak Investigations Using Oxford Nanopore Sequencing Technologies

Technologies, and we compared this to Illumina short-read-based metagenomics. After 12 h of MinION sequencing, strain-level characterization could be achieved, linking the food containing a pathogen to the ...

4DEMON: Integrating 40 Years of Data on PCB and Metal Contamination in Marine Sediments of the Belgian Part of the North Sea

changes in the marine environment. Whereas time series analyses based on monitoring data typically span one or two decades, this work aimed to integrate 40 years of monitoring and research data on ...

Intake of food supplements based on algae or cyanobacteria may pose a health risk due to elevated concentrations of arsenic species.

Keywords: Arsenic Food Supplements Speciation Abstract: Despite the health benefits of food supplements (FS) based on algae or cyanobacteria, the elevated arsenic (As) concentrations in these FS may raise ...

Whole Genome Sequencing Provides an Added Value to the Investigation of Staphylococcal Food Poisoning Outbreaks

of S. aureus isolates, including some belonging to a confirmed foodborne outbreak, its added value as the ultimate multiplexing method was demonstrated. In contrast to PCR-based se gene detection for ...

Evaluating SARS-CoV-2 spike and nucleocapsid proteins as targets for antibody detection in severe and mild COVID-19 cases using a Luminex bead-based assay.

severe Covid-19 cases developed a clear IgM and IgA response, titers fell below the detection threshold in more than 20 % of mild cases in our bead-based assay. In conclusion, our data supports the use of ...

Identification of the Source for Contamination of Carcasses in a Large Pig Slaughterhouse.

(18.3%) this slaughter step. In the unclean area, a limited number of oral cavity samples were positive after bleeding, while a dramatic increase of positives was observed after dehairing. was detected in ...

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