Zoekresultaten - 125 results

Investigation of a Possible Link Between Vaccination and the 2010 Sheep Pox Epizootic in Morocco.

phylogenetic analyses undertaken as part of this study showed significant differences between field isolates and the Moroccan vaccine strain. New PCR methods were developed to differentiate between wild-type ...

Complete Genome Sequence of a Vaccinal Newcastle Disease Virus Strain Isolated from an Owl (Rhinoptynx clamator).

A Newcastle disease virus (NDV) was isolated in chicken embryonated eggs after detection by real-time reverse transcription- PCR (RRT- PCR) from a captive owl swab. The complete genome sequence of ...

Development of a Luminex xTAG® assay for cost-effective multiplex detection of β-lactamases in Gram-negative bacteria.

of ESBL s and carbapenemases in Gram-negative bacilli. This assay had to be (i) superior to traditional (multiplex) PCR /sequencing-based tests in turn-around time, gene coverage and the ability to ...

Direct comparison of two vaginal self-sampling devices for the detection of human papillomavirus infections.

understand (p <0.001) and to use (p=0.002). DISCUSSION: This study confirms that hr- HPV testing with a clinically validated PCR-based HPV assay is as accurate on self-samples as on clinician-samples ...

Clinical and analytical performance of the PapilloCheck HPV-Screening assay using the VALGENT framework.

prevalence was stratified by age and cytology grade and compared with the luminex typing assay incorporating a GP5 +6+ PCR (GP5 +/6+ LMNX Assay). Clinical outcomes were compared with GP5 +/6+ EIA. RESULTS: ...

A Versatile Panel of Reference Gene Assays for the Measurement of Chicken mRNA by Quantitative PCR.

Reaction Reference Standards RNA, Messenger Abstract: Quantitative real-time PCR assays are widely used for the quantification of mRNA within avian experimental samples. Multiple stably-expressed reference ...

A Tale of Tails: Dissecting the Enhancing Effect of Tailed Primers in Real-Time PCR.

of inhibitors. In depth analysis of PCR products indeed revealed the rapid accumulation of forward-primer derived artefacts. More importantly, tailing of the forward primer delayed artefacts formation ...

Clinical problems due to encephalomyocarditis virus infections in two pig herds.

conventional gel-based polymerase chain reaction tests. The real-time PCR test that gave initially negative result was further optimized to avoid false negative results. CONCLUSIONS: Typical lesions are not ...

High Prevalence of Plasmodium falciparum Infection in Asymptomatic Individuals from the Democratic Republic of the Congo.

within asymptomatic carriers and that PCR assays are currently recognized as the most sensitive methods for Plasmodium identification, this study was conducted to weigh the asymptomatic carriage in DRC by ...

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