Zoekresultaten - 18 results
A more sensitive, efficient and ISO 17025 validated Magnetic Capture real time PCR method for the detection of archetypal Toxoplasma gondii strains in meat.
demonstrated. For this, a bioassay is still the reference method. As an alternative, various PCR methods have been developed, but due to the limited amount of sample that can be tested, combined with a low tissue ...
Molecular Subtyping of Salmonella Typhimurium with Multiplex Oligonucleotide Ligation-PCR (MOL-PCR).
Biol, Volume 1616, p.39-69 (2017) Abstract: A multiplex oligonucleotide ligation- PCR (MOL- PCR) assay is a valuable high-throughput technique for the detection of bacteria and viruses, for ...
Model-Based Classification for Digital PCR: Your Umbrella for Rain.
Clement, Lieven Source: Anal Chem, Volume 89, Issue 8, p.4461-4467 (2017) Abstract: Standard data analysis pipelines for digital PCR estimate the concentration of a target nucleic acid by digitizing the ...
Discrimination of three genetically close Aspergillus species by using high resolution melting analysis applied to indoor air as case study.
(PCR) and the real-time PCR are the most frequently used tools for indoor fungal detection. However, even if these tools have demonstrated their appropriate performance, some of them are not able to ...
Development of a Luminex xTAG® assay for cost-effective multiplex detection of β-lactamases in Gram-negative bacteria.
identification of ESBL s and carbapenemases in Gram-negative bacilli. This assay had to be (i) superior to traditional (multiplex) PCR /sequencing-based tests in turn-around time, gene coverage and the ability to ...
Prevalence and Genetic Diversity of Livestock-Associated Methicillin-Resistant Staphylococcus aureus on Belgian Pork.
colonies were confirmed using a MRSA-specific triplex PCR assay and a CC398-specific PCR assay. The isolates (n = 147) were further characterized by SCC mec typing, multiple-locus variable number tandem ...
A multiplex oligonucleotide ligation-PCR as a complementary tool for subtyping of Salmonella Typhimurium
INFECTION infections International investigation IS IT Laboratories LEVEL Luminex Marker Markers method microsphere array MOL-PCR Molecular molecular markers Network Objective observed ON outbreak outbreaks ...
Erratum to: Development and performance assessment of a qualitative SYBR® green real-time PCR assay for the detection of Aspergillus versicolor in indoor air.
Publication Type: Peer reviewed scientific article Authors: Libert, X; Camille Chasseur; Bladt, S; Ann Packeu; Bureau, F; Nancy Roosens; Sigrid C.J. De Keersmaecker Source: Appl Microbiol Biotechnol, Volume 99, Issue 19, p.8319-20 (2015) Health Topics: F ...
Current and new approaches in GMO detection: challenges and solutions
https://www.sciensano.be/nl/biblio/current-and-new-approaches-gmo-detection-challenges-and-solutions
DNA, have been developed to implement these legislations. Due to its numerous advantages, the quantitative PCR (qPCR) is the method of choice for the enforcement laboratories in GMO routine analysis. ...
Development and performance assessment of a qualitative SYBR® green real-time PCR assay for the detection of Aspergillus versicolor in indoor air.
excellent alternative for the monitoring of indoor fungal contaminations. As a case study, we developed a SYBR ® green real-time PCR-based assay for the specific detection and identification of Aspergillus ...