Zoekresultaten - 105 results
Detection and characterization of Brucella spp. in bovine milk in small-scale urban and peri-urban farming in Tajikistan.
milk in the urban and peri-urban area of Dushanbe, Tajikistan. Brucella DNA was detected in 10.3% of 564 cow milk samples by IS711-based real-time PCR. This finding is concerning because consumption of ...
Incorporation of the influenza A virus NA segment into virions does not require cognate non-coding sequences.
incorporation of the viral segments was tested by real-time RT- PCR. An initial virus growth deficiency correlated to a specific defect in NA segment incorporation. Upon serial passages, growth properties were ...
Schmallenberg Virus in Belgium: Estimation of Impact in Cattle and Sheep Herds.
D) were randomly selected. Farms from groups A (n = 53) and C (n = 42) received SBV-positive result at RT- PCR in the Belgian National Reference Laboratory (NRL). Farms from groups B (n = 29) and ...
Development of molecular tools for rapid detection and quantification of indoor airborne molds to assess their impact on public health
a specific ITS-based SYBR ®green real-time PCR (qPCR) assay for Aspergillus versicolor, a species frequently observed in indoor air and known to be allergenic. Additionally, an ITS-based qPCR assay was ...
Detection and Molecular Characterization of Foot and Mouth Disease Viruses from Outbreaks in Some States of Northern Nigeria 2013-2015.
FMD-affected animals using rRT- PCR, and cytopathic effect was found in cell culture by 59% (44 of 74) of these samples. Three FMDV serotypes O, A and SAT2 were isolated and characterized. The phylogenetic ...
Age-Dependent Differences in Pseudorabies Virus Neuropathogenesis and Associated Cytokine Expression.
in important clinical disease, although the associated morbidity and mortality were lower in older pigs. Quantitative PCR analysis of viral DNA in different organs confirmed the general knowledge on ...
Laboratory validation of two real-time RT-PCR methods with 5′-tailed primers for an enhanced detection of foot-and-mouth disease virus
Publication Type: Peer reviewed scientific article Authors: Frank Vandenbussche; David Lefebvre; Ilse De Leeuw; Steven Van Borm; Kris De Clercq Source: Journal of Virological Methods, Volume 246 (2017) Health Topics: Animal health Santé animale Diergezon ...
Investigation of a Possible Link Between Vaccination and the 2010 Sheep Pox Epizootic in Morocco.
phylogenetic analyses undertaken as part of this study showed significant differences between field isolates and the Moroccan vaccine strain. New PCR methods were developed to differentiate between wild-type ...
Complete Genome Sequence of a Vaccinal Newcastle Disease Virus Strain Isolated from an Owl (Rhinoptynx clamator).
A Newcastle disease virus (NDV) was isolated in chicken embryonated eggs after detection by real-time reverse transcription- PCR (RRT- PCR) from a captive owl swab. The complete genome sequence of ...
Direct comparison of two vaginal self-sampling devices for the detection of human papillomavirus infections.
understand (p <0.001) and to use (p=0.002). DISCUSSION: This study confirms that hr- HPV testing with a clinically validated PCR-based HPV assay is as accurate on self-samples as on clinician-samples ...