Zoekresultaten - 105 results
Clinical validation of hrHPV testing on vaginal and urine self-samples in primary cervical screening (cross-sectional results from the Papillomavirus Dumfries and Galloway-PaVDaG study).
papillomavirus (hrHPV) PCR-based assay to detect high-grade cervical intraepithelial neoplasia (CIN2 +) in self-collected vaginal and urine samples. SETTING: Women attending routine cervical screening in ...
Serodiagnosis of whooping cough in Belgium: results of the National Reference Centre for Bordetella pertussis anno 2013.
early diagnosis of the disease and PCR analysis on nasopharyngeal swabs is the method of choice during the first 2 weeks and always for young children <1 year old. For sera collected during the first ...
Investigation on possible transmission of monkeys' Plasmodium to human in a populations living in the equatorial rainforest of the Democratic republic of Congo.
near the rainforest of the Democratic Republic of Congo (DRC) where several species of non-human primates are living. Two successive real-time PCR s were identified in the literature and used in ...
VALGENT: A protocol for clinical validation of human papillomavirus assays.
laboratories in Antwerp (Belgium) and Edinburgh (Scotland), were completed. Two new assays (G5 +/6+ PCR- LMNX and Xpert HPV) were validated for screening by showing similar accuracy for cervical precancer as the ...
Evaluation of detection methods for non-O157 Shiga toxin-producing Escherichia coli from food.
in parallel in Buffered Peptone Water (BPW) and Brila broth. After enrichment, detection was performed using real-time PCR (qPCR), and isolation using two chromogenic agar media, CHROM agar™ STEC and ...
A molecular approach for the rapid, selective and sensitive detection of Exophiala jeanselmei in environmental samples: development and performance assessment of a real-time PCR assay
jeanselmei. In this study, we developed a SYBR ®green real-time PCR assay based on the internal transcribed spacer 2 from the 18S ribosomal DNA complex for the specific detection of E. jeanselmei. The ...
Toxoplasma gondii in stranded marine mammals from the North Sea and Eastern Atlantic Ocean: Findings and diagnostic difficulties
Otters PCR Pinnipedia polymerase chain reaction Seroepidemiologic Studies Seroprevalence Toxoplasma Toxoplasma gondii Toxoplasmosis, Animal Abstract: The occurrence of the zoonotic protozoan parasite ...
Optimized MOL-PCR for Characterization of Microbial Pathogens
surveillance, outbreak detection, and tracing of outbreak sources. This unit describes a multiplex oligonucleotide ligation- PCR (MOL- PCR) optimized for characterization of microbial pathogens. With MOL- PCR ...
Prevalence and Genetic Diversity of Livestock-Associated Methicillin-Resistant Staphylococcus aureus on Belgian Pork.
colonies were confirmed using a MRSA-specific triplex PCR assay and a CC398-specific PCR assay. The isolates (n = 147) were further characterized by SCC mec typing, multiple-locus variable number tandem ...
Use of next generation sequencing data to develop a qPCR method for specific detection of EU-unauthorized genetically modified Bacillus subtilis overproducing riboflavin.
TaqMan® qPCR method, specifically detecting this GM vitamin B2 overproducing bacterium. The method complies with EU critical performance parameters for specificity, sensitivity, PCR efficiency and ...