Zoekresultaten - 105 results
Which high-risk HPV assays fulfil criteria for use in primary cervical cancer screening?
expert team in 2009, based on reproducibility and relative sensitivity and specificity compared to Hybrid Capture-2 or GP5 +/6+ PCR-enzyme immunoassay. These latter two hrHPV DNA assays were validated in ...
Development and performance assessment of a qualitative SYBR® green real-time PCR assay for the detection of Aspergillus versicolor in indoor air.
excellent alternative for the monitoring of indoor fungal contaminations. As a case study, we developed a SYBR ® green real-time PCR-based assay for the specific detection and identification of Aspergillus ...
First isolation, identification, phenotypic and genotypic characterization of Brucella abortus biovar 3 from dairy cattle in Tanzania.
with the RBT. Three Brucella were isolated from an aborted fetus and associated membranes. Real time PCR (IS711), Bruce-ladder and classical biotyping classified the isolates as B. abortus biovar 3. ...
Statistical framework for detection of Genetically Modified Organisms based on Next Generation Sequencing
number and diversity of genetically modified (GM) crops has significantly increased, their analysis based on real-time PCR (qPCR) methods is becoming increasingly complex and laborious. While several ...
European interlaboratory comparison of Schmallenberg virus (SBV) real-time RT-PCR detection in experimental and field samples: The method of extraction is critical for SBV RNA detection in semen.
inhibitory effects of semen components on PCR results. All methods used by the 44 laboratories were sufficiently robust to detect SBV RNA with high diagnostic sensitivity (100%) and specificity (95.8%) in all ...
Detection and Isolation of Swine Influenza A Virus in Spiked Oral Fluid and Samples from Individually Housed, Experimentally Infected Pigs: Potential Role of Porcine Oral Fluid in Active Influenza A Virus Surveillance in Swine.
period in nasal swab samples. Therefore, more sensitive diagnostic methods to monitor circulating virus strains are requisite. METHODS: qRT- PCR and virus isolations were performed on oral fluid and nasal ...
Guidelines for optimisation of a multiplex oligonucleotide ligation-PCR for characterisation of microbial pathogens in a microsphere suspension array.
PCR (MOL- PCR) different molecular markers can be simultaneously analysed in a single assay and high levels of multiplexing can be achieved in high-throughput format. As such, MOL- PCR is a convenient ...
Risk of Misinterpretation of Ebola Virus PCR Results After rVSV ZEBOV-GP Vaccination.
Publication Type: Peer reviewed scientific article Authors: Cnops, Lieselotte; Gerard, Michèle; Vandenberg, Olivier; Van den Wijngaert, Sigi; Heyndrickx, Leo; Willems, Elisabeth; Demeulemeester, Kathy; De Clerck, Hilde; Dediste, Anne; S. Callens; De Munte ...
Application of MOL-PCR to replace Salmonella phage typing
Publication Type: Scientific poster, presentation or proceeding Authors: Wuyts,V. Source: Doctoral Seminar, NA, Issue WIV-ISP, NA (2015) Keywords: application Luminex MOL-PCR Salmonella Health ...
Extensive genetic variability linked to IS26 insertions in the fljB promoter region of atypical monophasic variants of Salmonella enterica serovar Typhimurium.
The genetic differences between these strains and phenotypically biphasic Salmonella Typhimurium were analyzed through PCR and DNA sequencing. Genetic alterations in the fljB promoter region affecting ...