Zoekresultaten - 115 results

A duplex real-time RT-PCR for the detection of bluetongue virus in bovine semen.

Keywords: Animals Bluetongue Bluetongue virus Cattle Cattle Diseases Reproducibility of Results Reverse Transcriptase Polymerase Chain Reaction RNA, Viral semen Sensitivity and Specificity virology Abstract: ...

Simultaneous detection of bluetongue virus RNA, internal control GAPDH mRNA, and external control synthetic RNA by multiplex real-time PCR.

insect-borne disease of domestic and wild ruminants that requires strict monitoring by sensitive, reproducible and robust methods. Real-time reverse transcription polymerase chain reaction (RT-qPCR) analysis has ...

Direct detection and genotyping of Toxoplasma gondii in meat samples using magnetic capture and PCR36884

Magnetics Meat method methods national Netherlands ON Parasites parasitology PCR Pig Pigs polymerase chain reaction prevention prevention strategies public public health Public-health Quantification ...

Use of pJANUS-02-001 as a calibrator plasmid for Roundup Ready soybean event GTS-40-3-2 detection: an interlaboratory trial assessment.

constructed. The dynamic range, efficiency and limit of detection for the soybean event GTS-40-3-2 real-time quantitative polymerase chain reaction (Q- PCR) system described by Terry et al. (J AOAC Int ...

Fine quantification of avian influenza H5N1 escape mutant quasispecies populations using mutation-specific real-time PCR.

Adaptation, Physiological Animals Chick Embryo Evolution, Molecular Gene Expression Regulation, Viral Genes, Viral Influenza A Virus, H5N1 Subtype Mutation polymerase chain reaction RNA, Viral Sequence ...

Rapid detection of Eurasian and American H7 subtype influenza A viruses using a single TaqManMGB real-time RT-PCR.

Chain Reaction Specific Pathogen-Free Organisms Abstract: A real-time reverse transcription PCR (RRT- PCR) targeting a highly conserved HA2 H7 region was developed for the detection of all H7 subtype ...

First epidemiological study on exposure to Neospora caninum in different canine populations in the Algiers District (Algeria)14

purification journal LEVEL Medicine methods n Neospora observed ON ORIGIN parameters parasitology PCR polymerase chain reaction POPULATION Populations prevalence proportion real time PCR region Research Research ...

Multiplex real-time RT-PCR for simultaneous detection of GI/GII noroviruses and murine norovirus 136839

1 (MNV-1) real-time PCR detection assay described recently was integrated successfully into the multiplex assay, making it possible to detect GI and GII NoVs and MNV-1 in one reaction tube with MNV-1 plasmid ...

Validation of two real-time RT-PCR methods for foot-and-mouth disease diagnosis: RNA-extraction, matrix effect, uncertainty of measurement and precision.

Specificity Abstract: Real-time reverse transcription polymerase chain reaction (rRT- PCR) assays are being used routinely for diagnosing foot-and-mouth disease virus (FMDV). Although most laboratories ...

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