Zoekresultaten - 46 results

Strain-level characterization without culture enrichment? Easing and accelerating outbreak investigation using shotgun metagenomics facilitated with nanopore adaptive sampling

adaptive sampling, following whole genome amplification to increase the amount of genetic material to meet sequencing standards. The strain-level data analysis was performed as previously described (Buytaers ...

Development of strain-level shotgun metagenomics approaches to detect and characterize microbiological contaminants in the context of food safety

chosen for further studies (i.e. 24 hours enrichment as stated in the current international standard procedure, classical commercial DNA extraction and no amplification). Cheese samples were spiked with ...

Microbiological safety of aged meat.

microbiological hazards and spoilage bacteria was examined and current practices are described. As ‘standard fresh’ and wet-aged meat use similar processes these were differentiated based on duration. In addition ...

Report on an initial assessment of long-read sequencing for metagenome (community and AMR) analysis (defined community, spiked and real simple and complex matrices) and comparison to current short-read standard

Publication Type: Sci. report, recommendat°, guidance doc., directive, monograph Authors: Sigrid C.J. De Keersmaecker; Mathieu Gand; Indre Navickaite; Josephine Gruetzke; Lee-Julia Bartsch; Saria Otani; Giuliano Garofolo; Marco Di Domenico; Valeria Michel ...

Methodological choices in brucellosis burden of disease assessments: A systematic review

A systematic search for brucellosis burden of disease calculations was conducted in pre-selected international and grey literature databases. Using a standardized reporting framework, we evaluated each estimate ...

Development of a taxon-specific real-time PCR method targeting the Bacillus subtilis group to strengthen the control of genetically modified bacteria in fermentation products

complying with the Minimum Performance Requirements for Analytical Methods of GMO Testing that is used as a standard by the GMO enforcement laboratories. The method’s applicability was also tested on 25 ...

Providing Measurement, Evaluation, Accountability, and Leadership Support (MEALS) for Non-communicable Diseases Prevention in Ghana: Project Protocol

and pupils of Ghanaian basic schools. Results: The study establishes a standardized approach to providing implementation science evidence for the prevention of non-communicable diseases (NCD s) in ...

Sensitivity of candling as routine method for the detection and recovery of ascaridoids in commercial fish fillets.

reference standard. The overall Se of candling was 31% (95% CI 23-41%) and NPV was 87% (95% CI 85-90%). The Se increased with higher numbers of larvae/100 g infected muscle. A low NPV was found for the belly ...

In Vivo Kinetics and Biotransformation of Aflatoxin B1 in Dairy Cows Based on the Establishment of a Reliable UHPLC-MS/MS MethodDataSheet1.docx

successfully validated by the determination of linearity (R2  ≥ 0.990), sensitivity (lower limit of quantification, 0.1–0.2 ng ml−1), recovery (79.5–111.2%), and precision relative standard deviation (RSD) ...

Ten-year changes in diet quality among adolescents and young adults (Food Consumption Survey 2004 and 2014, Belgium).

nutritional quality of adolescent and young adult’s diet, as measured by the modified Nutrient Profiling System of the British Food Standards Agency individual Dietary Index (FSA m- NPS- DI) which funds the ...

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