Zoekresultaten - 14 results

Nanopore sequencing technology: a new route for the fast detection of unauthorized GMO.

region. This DNA walking is performed on transgenic elements, commonly found in GMO, that were earlier detected by real-time PCR (qPCR) screening. Previously, we have demonstrated the ability of this ...

Development and validation of an integrated DNA walking strategy to detect GMO expressing cry genes

of interest, including their transgene flanking regions and the unnatural associations of elements in their transgenic cassettes. To this end, the p35S, tNOS and t35S pCAMBIA elements have been ...

An integrated strategy combining DNA walking and NGS to detect GMO

strategy in routine GMO analysis. In all tested samples, the presence of multiple GMO s was unambiguously proven by the characterization of transgene flanking regions and the combinations of elements that ...

Scientific opinion on Malachite green in food

and/or mice, and of micronuclei in mice. LMG also induced cII transgene mutations in mouse liver. MG caused a small, not dose-related, increase in thyroid gland follicular adenomas and carcinomas, and of ...

Field trials with GM trees: a step-by-step approach

Publication Type: Scientific book or chapter Authors: D. C.M. Glandorf; Didier Breyer Source: Biosafety of forest transgenic trees- improving the scientific basis for safe tree development and ...

Strategies to detect unauthorized GMO in the food and feed chain (PhD thesis)

alia on elements found in their transgenic cassette. Second, according to this information, key targets, frequently found in GMO (p35S and tNOS) or exclusively observed in EU unauthorized GMO (t35S ...

Biotech Rice: Current developments and future detection challenges in food and feed chain

& Technology, Volume 52, Number 79 (2016) Keywords: Biotech crop detection Transgenic Rice Abstract: Background To improve agricultural practices and the food/feed security, plant breeding techniques ...

Regulatory T-cell development and function are impaired in mice lacking membrane expression of full length intercellular adhesion molecule-1.

Female Gene Expression Intercellular Adhesion Molecule-1 Lymphocyte Activation mice Mice, Transgenic T-Lymphocyte Subsets T-Lymphocytes, Regulatory Thymocytes Abstract: To further investigate the ...

Integrated DNA walking system to characterize a broad spectrum of GMOs in food/feed matrices.

methods targeting p35S or tNOS, the most common transgenic elements found in GM crops, were developed. These newly developed DNA walking methods are completing the previously implemented DNA walking method ...

Statistical framework for detection of Genetically Modified Organisms based on Next Generation Sequencing

predict the number of NGS reads needed to detect transgene sequences, to prove their integration into the host genome and to identify the specific transgene event in a sample with known composition. This ...

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