Zoekresultaten - 71 results

Validation of two commercial real-time RT-PCR kits for rapid and specific diagnosis of classical swine fever virus.

147, Issue 1, p.136-42 (2008) Keywords: Animals Classical Swine Fever Classical swine fever virus Reproducibility of Results Reverse Transcriptase Polymerase Chain Reaction Sensitivity and Specificity ...

An international inter-laboratory ring trial to evaluate a real-time PCR assay for the detection of bovine herpesvirus 1 in extended bovine semen.

a good substitute for the slow and laborious virus isolation, for the screening testing at artificial insemination centres and for international trade. Health Topics:  Animal health Category:  A1 FOS ...

Validation of a real-time PCR assay for the detection of bovine herpesvirus 1 in bovine semen.

conventional virus isolation method for the detection of BoHV-1 in extended semen. The specificity of the assay is as expected. The assay had an analytical sensitivity of 0.38 TCID (50) virus spiked into ...

A universal avian endogenous real-time reverse transcriptase-polymerase chain reaction control and its application to avian influenza diagnosis and quantification.

avian endogenous internal control (bird beta-actin) and apply it to influenza A diagnosis. Moreover, this internal control proves useful as a normalizer control for virus quantification, because ...

Lethality and molecular characterization of an HPAI H5N1 virus isolated from eagles smuggled from Thailand into Europe.

smuggled into Europe from Thailand were seized at Brussels International Airport. A highly pathogenic avian influenza virus, denominated A/crested eagle/Belgium/0½004, was isolated from these birds and ...

Bluetongue virus detection by two real-time RT-qPCRs targeting two different genomic segments.

Keywords: Animals Bluetongue Bluetongue virus Cell Line Cricetinae DNA Primers DNA Probes Genome, Viral Nucleic Acid Amplification Techniques Reproducibility of Results Reverse Transcriptase Polymerase Chain ...

Avian influenza: mini-review, European control measures and current situation in Asia.

contribute to the dissemination of the virus. In the context of H5N1, all movements (trade, high international mobility, migration and smuggling) can become high risk factors of spreading the disease. In most ...

Binding and entry characteristics of porcine circovirus 2 in cells of the porcine monocytic line 3D4/31.

/31 were studied by visualization of binding and internalization of PCV2 virus-like particles (VLP s) by confocal microscopy and chemical inhibition of endocytic pathways (clathrin- and ...

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