Zoekresultaten - 78 results
Rapid method for the confirmatory analysis of chrysoidine in aquaculture products by ultra-performance liquid chromatography-tandem mass spectrometry.
water and an acetonitrile gradient within 6 min. Mass spectrometric analysis was performed in the positive electrospray MS / MS mode. The limit of quantification was 0.25 ng/g, which was 30 times lower ...
Revisiting the loading dose of amikacin for patients with severe sepsis and septic shock90
elimination half-life, 4.6 (3.2 to 7.8) hours; and total clearance, 1.98 (1.28 to 3.54) ml/min/kg. No correlation was found between the amikacin peak and any clinical or hemodynamic variable. CONCLUSIONS: As ...
Membrane permeabilization and cellular death of Escherichia coli, Listeria monocytogenes and Saccharomyces cerevisiae as induced by high pressure carbon dioxide treatment.
treatment time (0-60 min) was examined. Loss of membrane integrity was measured as increased uptake of the fluorescent dye propidium iodide (PI) with spectrofluorometry, while cell inactivation was determined ...
A proposed validation method for automated nucleic acid extraction and RT-qPCR analysis: an example using Bluetongue virus.
extract 186 samples in 2h and 30 min. The automation of the extraction protocol implied some additional validation parameters to be determined such as position-effect, absence of cross-contamination and ...
Bioaugmentation of microbial communities in laboratory and pilot scale sequencing batch biofilm reactors using the TOL plasmid.
degradation rate was enhanced after donor inoculation from 0.98 mg BA /min prior to inoculation to 1.9 mg BA /min on the seventeenth day of operation. Survival of a bioaugmented strain, conjugative plasmid ...
Optimization of the blanching process to reduce acrylamide in fried potatoes
temperature was the main influencing factor for sugar extraction and subsequent acrylamide mitigation. Blanching at temperatures of about 70 °C for a short period of time (about 10 min) was more efficient ...
Quantification of gene expression of Listeria monocytogenes by real-time reverse transcription PCR: optimization, evaluation and pitfalls36791
(RNA queous-Micro kit was found to be the most sensitive RNA isolation kit. For cDNA synthesis, the use of random hexamers and an incubation time of 90 min with the Multiscribe RT-enzyme resulted in the ...
Kinetic and biologic properties of recombinant ChIFN-gamma expressed via CELO-virus vector.
vector was able to replicate in vitro in the loghorn male hepatoma (LMH) hepatocyte cell line and to produce detectable levels of recombinant cytokine in supernatant as early as 90 min post-infection. ...
Survival and stress induced expression of groEL and rpoD of Campylobacter jejuni from different growth phases36763
of heat shock (55 degrees C, 3 min) and oxidative stress (3 mM H2O2 for 10 min or prolonged incubation at atmosphere oxygen concentration) on non-starved and starved cells of Campylobacter jejuni from ...
Biological control of the size and reactivity of catalytic Pd(0) produced by Shewanella oneidensis.
effective electron donor for bioreductive detoxification of Pd(II), since there was a 2 log reduction of culturable cells and a 20% decrease of viable cells within 60 min, followed by a slow recovery. When ...