Zoekresultaten - 15 results

Development of strain-level shotgun metagenomics approaches to detect and characterize microbiological contaminants in the context of food safety

It was proven that whole genome sequencing has a higher resolution than any combination of tests carried out so far. But the isolation is not always possible, and it is time-consuming. A new method ...

Development, validation and application of multi-class methods for the analysis of food additives by liquid chromatography coupled to tandem mass spectrometry

aimed to develop an accurate and high-throughput analytical method enabling the simultaneous determination of additives from different functional classes to facilitate controls and generate occurrence ...

Development of a genoserotyping system for the identification of Salmonella serotypes

was used to develop in addition a real-time PCR as an alternative method for the rapid identification of S. Paratyphi B and the determination of its variant Java, replacing a complex and subjective ...

A more sensitive, efficient and ISO 17025 validated Magnetic Capture real time PCR method for the detection of archetypal Toxoplasma gondii strains in meat.

demonstrated. For this, a bioassay is still the reference method. As an alternative, various PCR methods have been developed, but due to the limited amount of sample that can be tested, combined with a low tissue ...

Development and performance assessment of a luminex xMAP® direct hybridization assay for the detection and identification of indoor air fungal contamination.

dead and uncultivable fraction. Moreover, culture-based protocols are often time-consuming. In this context, molecular tools are a powerful alternative, especially those allowing multiplexing. In this ...

Discrimination of three genetically close Aspergillus species by using high resolution melting analysis applied to indoor air as case study.

therefore important. To avoid problems linked to culture-dependent protocols, molecular methods are increasingly being proposed as an alternative. Among these molecular methods, the polymerase chain reaction ...

Construction of Nontoxigenic Mutants of Nonproteolytic Clostridium botulinum NCTC 11219 by Insertional Mutagenesis and Gene Replacement.

can grow and produce botulinum neurotoxin during refrigerated storage. The principles of control of gIICb by conventional food processing and preservation methods have been well investigated and ...

Evaluation of the migration of chemicals from baby bottles under standardised and duration testing conditions.

bottles in the European Union (EU), alternative materials, such as polypropylene, polyethersulphone, Tritan™ copolyester, etc., have appeared on the market. Based on an initial screening and in vitro ...

Current and new approaches in GMO detection: challenges and solutions

properties. To address these challenges, alternative GMO detection methods have been developed, allowing faster detections of single GM target (e.g., loop-mediated isothermal amplification), simultaneous ...

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