Zoekresultaten - 119 results
Retrospective surveillance of viable Bacillus cereus group contaminations in commercial food and feed vitamin B2 products sold on the Belgian market using whole-genome sequencing
(100%) and fosfomycin (88.89%), and a subset was predicted to be resistant to streptothricin (30.56%). Phylogenomic analysis revealed that some isolates obtained from different products were closely ...
BSFM 2023- L. monocytogenes in Plant-Based Foods: insights from of a recent foodborne outbreak linked to vegan alternative to cheese
was performed for clustering analysis. Discussion Due to the growing demand for novel and alternative food products among the population, concerns are raised about the potential risks associated with ...
Strain-level characterization without culture enrichment? Easing and accelerating outbreak investigation using shotgun metagenomics facilitated with nanopore adaptive sampling
resolved. Shotgun metagenomics is a sequencing method involving the analysis of the genetic material directly extracted from a sample, without the need for an isolation of the bacteria. By sequencing and ...
Development of strain-level shotgun metagenomics approaches to detect and characterize microbiological contaminants in the context of food safety
Ultimately, a validation of the method is still necessary in order to obtain a precise limit of detection based on the analysis of a large dataset of samples. Moreover, other technologies can still be ...
Comparison of 6 DNA extraction methods for isolation of high yield of high molecular weight DNA suitable for shotgun metagenomics Nanopore sequencing to detect bacteriaAbstractBackgroundResultsConclusion
improves the reconstruction of genomes and helps to resolve complex genomic contexts, especially in the case of metagenome analysis. To take the best advantage of long-read sequencing, DNA extraction methods ...
Simultaneous Detection and Quantification of Aflatoxin M1, Eight Microcystin Congeners and Nodularin in Dairy Milk by LC-MS/MS
extracted with methanol 80%, followed by an SPE clean-up step before LC- MS / MS analysis. The LOQ was fixed at 1 µg/L for the nine cyanotoxins and 0.05 µg/L for AFM1. Recoveries were measured between 82.67% ...
The contribution of cacao consumption to the bioaccessible dietary cadmium exposure in the Belgian population
the Belgian population, in combination with ICP- MS analysis of a large subset of cacao-containing products (N=349). Both the average chocolate consumption (28 g day-1) and the relative contribution of ...
Targeted High-Throughput Sequencing Enables the Detection of Single Nucleotide Variations in CRISPR/Cas9 Gene-Edited Organisms
propose a targeted high-throughput sequencing approach, including (i) a prior PCR-based enrichment step to amplify regions of interest, (ii) a sequencing step, and (iii) a data analysis methodology to ...
Aluminium intake through the consumption of selected baby foods and risk characterization in a population of Brazilian infants aged 0 to 36 months
Antônio Morgano; Arisseto-Bragotto, Adriana Pavesi Source: Journal of Food Composition and Analysis, Volume 115 (2023) Keywords: Aluminium Food Safety Risk Assessment Abstract: Aluminium (Al) dietary intake ...
Report on an initial assessment of long-read sequencing for metagenome (community and AMR) analysis (defined community, spiked and real simple and complex matrices) and comparison to current short-read standard
Publication Type: Sci. report, recommendat°, guidance doc., directive, monograph Authors: Sigrid C.J. De Keersmaecker; Mathieu Gand; Indre Navickaite; Josephine Gruetzke; Lee-Julia Bartsch; Saria Otani; Giuliano Garofolo; Marco Di Domenico; Valeria Michel ...