Zoekresultaten - 10 results
Development of strain-level shotgun metagenomics approaches to detect and characterize microbiological contaminants in the context of food safety
have always been analyzed and characterized after isolation. Since the advent of second generation sequencing, it has been possible to characterize the complete genome of these isolates to the SNP level. ...
Comparison of 6 DNA extraction methods for isolation of high yield of high molecular weight DNA suitable for shotgun metagenomics Nanopore sequencing to detect bacteriaAbstractBackgroundResultsConclusion
improves the reconstruction of genomes and helps to resolve complex genomic contexts, especially in the case of metagenome analysis. To take the best advantage of long-read sequencing, DNA extraction methods ...
Report on an initial assessment of long-read sequencing for metagenome (community and AMR) analysis (defined community, spiked and real simple and complex matrices) and comparison to current short-read standard
applied genomics Manuscript versions: DOI: https://doi.org/10.5281/zenodo.7429361 Full text language: English Category: Scientific reports, recommendations, guidance documents, directives and ...
Shotgun metagenomics as a ONE Health tool for better protecting human health
microbiology. Compared to short read sequencing (Illumina), this technology allows to unambiguously detect and scaffold microbial genes to their host chromosomes, even for complex metagenomics samples, allowing ...
Development of a genoserotyping system for the identification of Salmonella serotypes
a number of 100 genomes used for comparisons) and bioinformatics tools such as Gegenees and BioNumerics. This marker selection was particularly complex for the detection of the heterogeneous population of S. ...
Strategies to detect unauthorized GMO in the food and feed chain (PhD thesis)
https://www.sciensano.be/nl/biblio/strategies-detect-unauthorized-gmo-food-and-feed-chain-phd-thesis
enforcement laboratories is becoming complex due mainly to the increasing number and diversity of GMO. To cope with the problematic of EU unauthorized GMO, this PhD aims to improve and strengthen the existing ...
Strategies to detect unauthorized genetically modified organisms in food and feed chain
(EU). However, the implementation of these legislations by the enforcement laboratories is becoming complex due mainly to the increasing number and diversity of GMO. To cope with the problematic of EU ...
Biotech Rice: Current developments and future detection challenges in food and feed chain
choice, regulatory frameworks were established in many countries around the world, such as in Europe. Their implementations, including detection systems usually based on qPCR, are becoming complex and ...
Integrated DNA walking system to characterize a broad spectrum of GMOs in food/feed matrices.
sample containing a low amount of the GM MON863 event, representing a more complex matrix in terms of genome size and sensitivity. Finally, to illustrate its applicability in GMO routine analysis by ...
Statistical framework for detection of Genetically Modified Organisms based on Next Generation Sequencing
number and diversity of genetically modified (GM) crops has significantly increased, their analysis based on real-time PCR (qPCR) methods is becoming increasingly complex and laborious. While several ...