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Zoekresultaten - 8 results

Towards Real-Time and Affordable Strain-Level Metagenomics-Based Foodborne Outbreak Investigations Using Oxford Nanopore Sequencing Technologies

related human isolate of the affected patient, by means of a single-nucleotide polymorphism (SNP)-based phylogeny. The inferred strain harbored the same virulence genes as the spiked isolate and could be ...

Whole Genome Sequencing Provides an Added Value to the Investigation of Staphylococcal Food Poisoning Outbreaks

C.J. De Keersmaecker Source: Frontiers in Microbiology, Volume 12 (2021) Keywords: DNA extraction kits enterotoxin gene profiling outbreak investigation relatedness determination Staphylococcus aureus, ...

Staphylococcal enterotoxin gene cluster: prediction of enterotoxin (SEG and SEI) production and of the source of food poisoning based on Saβ typing.

using commercially available kits.Six genes (and), encoding putative and undetectable enterotoxins, are located on the enterotoxin gene cluster () which is part of the genomic island Saβ. These ...

NAuRA: Genomic Tool to Identify Staphylococcal Enterotoxins in Strains Responsible for FoodBorne Outbreaks.

Hristo Daskalov; Michel-Yves Mistou; Jacques-Antoine Hennekinne Source: Front Microbiol, Volume 11 (2020) Keywords: bioinformatics tool; gene detection genomic staphylococcal enterotoxins genes variant ...

Detection and discrimination of five E. coli pathotypes using a combinatory SYBR® Green qPCR screening system

genes. uidA controls for E. coli presence and all the other genes are specific targets of E. coli pathotypes. For each gene, two primer pairs have been designed to guarantee a sufficient detection even in ...

Food-Borne Outbreak Investigation and Molecular Typing: High Diversity of Staphylococcus aureus Strains and Importance of Toxin Detection

pulsed-field gel electrophoresis (PFGE) and enterotoxin gene typing, confirmed the link between patients and the suspected foodstuffs. This also demonstrated the high diversity of CPS isolates both in the cases ...

Comparison of Droplet Digital PCR and qPCR for the Quantification of Shiga Toxin-Producing Escherichia coli in Bovine Feces.

Issue 5 (2016) Keywords: Animals Cattle Feces Genes, Bacterial polymerase chain reaction Shiga-Toxigenic Escherichia coli virulence Abstract: Cattle are considered to be the main reservoir for Shiga ...

RNA sequencing-based transcriptional overview of xerotolerance in Cronobacter sakazakii SP291.

RNA-seq data show that about 22% of the total  C.  sakazakii  genes were significantly upregulated and 9% were downregulated during desiccation survival. When reverse transcription-quantitative PCR (qRT- ...

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