Zoekresultaten - 95 results

Development, validation and evaluation of added diagnostic value of a q(RT)-PCR for the detection of genotype A strains of small ruminant lentiviruses.

Publication Type: Peer reviewed scientific article Authors: Nick De Regge; Ann Brigitte Cay Source: J Virol Methods, Volume 194, Issue 1-2, p.250-7 (2013) Keywords: Animals Belgium Goat Diseases ...

Development and validation of three Capripoxvirus real-time PCRs for parallel testing.

Publication Type: Peer reviewed scientific article Authors: Andy Haegeman; Zro, K; Frank Vandenbussche; Demeestere, L; Willem Van Campe; Ennaji, M M; Kris De Clercq Source: J Virol Methods, Volume ...

Effect of saliva stabilisers on detection of porcine reproductive and respiratory syndrome virus in oral fluid by quantitative reverse transcriptase real-time PCR.

Abstract: This study evaluated the effect of extraction-amplification methods, storage temperature and saliva stabilisers on detection of porcine reproductive and respiratory syndrome virus (PRRSV) RNA by ...

Characterization of methicillin-resistant Staphylococcus aureus from healthy carrier chickens.

livestock including poultry, little is known about the epidemiology of MRSA in broiler and layer chickens. We therefore investigated 372 poultry farms in Belgium. We also compared the isolation method ...

The origin of biased sequence depth in sequence-independent nucleic acid amplification and optimization for efficient massive parallel sequencing.

a 3’ random part used to prime complementary DNA synthesis and a 5’ defined tag sequence for subsequent amplification. Recently, this amplification method was combined with next generation sequencing to ...

In vitro and in vivo infectious potential of coxiella burnetii: a study on Belgian livestock isolates.

in the Netherlands during the 2007-2010 outbreaks. Infective doses were established with ethidium monoazide- PCR for the first time here applied to C. burnetii. This method allowed for the preparation ...

Importance of identification and typing of Brucellae from West African cattle: a review.

international classification methods with no time limit and no language restrictions. Studies reporting results of identification at genus level only were not considered for this review. This review revealed that ...

Influence of isolation methods on the occurrence of plasmid-carrying Yersinia enterocolitica serotype O:3 in slaughter pig tonsils, faeces and carcass surface swabs.

carcass samples were contaminated below the detection limit of enumeration. The relative sensitivity of the methods to recover pathogenic Y. enterocolitica varied among the different matrices. Nevertheless, ...

Intra-host variation structure of classical swine fever virus NS5B in relation to antiviral therapy.

alternative method of fighting the disease. However, classical swine fever virus is an RNA virus which presents a challenge as intra-host variation and the error prone RNA dependent RNA polymerase (RdRp) could ...

Bayesian estimation of the true prevalence, sensitivity and specificity of the Rose Bengal and indirect ELISA tests in the diagnosis of bovine brucellosis.

Specificity Serologic Tests Serotyping Abstract: Serology is the most convenient method for detecting brucellosis but the efficient use of such tests in disease control requires evaluation of diagnostic ...

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