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Zoekresultaten - 74 results

Viral load of high-risk human papillomaviruses as reliable clinical predictor for the presence of cervical lesions

undetermined significance (n = 100), low-grade squamous intraepithelial lesions (LSIL; n = 100), and high-grade squamous intraepithelial lesions (HSIL; n = 97) using type-specific multiplex quantitative ...

Serological profiles in nursery piglets colonized with Staphylococcus aureus.

Twenty-four out of the 31 piglets were challenged with MRSA ST398. The serum samples were analyzed for IgG antibodies to 39 S. aureus antigens, using a multiplex bead-based assay (xMAP technology, Luminex ...

A new type of multiplex assay to screen for GMO in food and feed samples developed within the ORIENT-EXPRESS project

food GMO MO Multiplex assay ORIENT-EXPRESS project Sample Samples Type Abstract: No abstract Service:  Activités transversales en génomique appliquée Transversal activities in applied genomics ...

Design of multiplex assays for molecular subtyping of pathogens with the Luminex xMAP technology

IT limitation Limitations Luminex microorganism microorganisms Molecular molecular subtyping Multiplex assay Oligonucleotides outbreak outbreaks pathogen reducing regulation Sample SOFTWARE specificity ...

Towards a Pathogenic Escherichia coli Detection Platform Using Multiplex SYBR(R)Green Real-Time PCR Methods and High Resolution Melting Analysis418

permitting the multiplex application in order to minimize the need of material and to allow for high-throughput analysis. In addition, High Resolution Melting (HRM) analysis allowing the discrimination among ...

Detection of C. botulinum neurotoxins A and B in milk by ELISA and immuno-PCR at higher sensitivity than mouse bio-assay.36466

toxin Abstract: Using commercially available antibodies and toxoids as templates, an ELISA, immuno-quantitative PCR (iqPCR), and multiplex immuno- PCR (iPCR) were developed for detection of Clostridium ...

Development and inter-laboratory validation study of an improved new real-time PCR assay with internal control for detection and laboratory diagnosis of African swine fever virus.

virology Abstract: A real-time polymerase chain reaction (PCR) assay for the rapid detection of African swine fever virus (ASFV), multiplexed for simultaneous detection of swine beta-actin as an endogenous ...

Nucleotide polymorphism-based single-tube test for robust molecular identification of all currently described Brucella species.

methods described during the last 20 years to identify Brucella, multiplexed amplification methods offer the cheapest and simplest technical solution for molecular identification. However, one disadvantage ...

Toxoplasma gondii and Neospora caninum in wildlife: common parasites in Belgian foxes and Cervidae?

bred deer samples positive for N. caninum. Brain samples from wild roe deer, red deer and red foxes (Vulpes vulpes) were tested for the presence of T. gondii and N. caninum DNA using multiplex real-time ...

Simultaneous detection of bluetongue virus RNA, internal control GAPDH mRNA, and external control synthetic RNA by multiplex real-time PCR.

RT-qPCR failure due to poor sample quality, improper nucleic acid extraction and/or PCR inhibition. To increase the diagnostic capacity and reduce costs, it is recommended to use a multiplex strategy which ...

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