Zoekresultaten - 14 results

Replication-Deficient Particles: New Insights into the Next Generation of Bluetongue Virus Vaccines.

and validate the suitability of these virus strains as the next-generation vaccines for BTV. IMPORTANCE: Bluetongue (BT) is a debilitating and in many cases lethal disease that affects ruminants of ...

Next-generation sequencing workflows in veterinary infection biology: towards validation and quality assurance.

This paper defines the potential diagnostic value and purposes of next-generation sequencing (NGS) applications in veterinary infection biology and explores their compatibility with the existing ...

Using genomics for surveillance of veterinary infectious agents

Keywords: Genomics high-throughput sequencing Metagenomics Next-generation sequencing Surveillance typing whole-genome sequencing Health Topics:  Animal health Santé animale Diergezondheid Manuscript ...

Next-generation sequencing shows West Nile virus quasispecies diversification after a single passage in a carrion crow (Corvus corone) in vivo infection model.

vector. This study investigated genetic diversification of WNV in avian hosts during a single passage using next-generation sequencing. Wild-captured carrion crows were subcutaneously infected using ...

Extensive genetic variability linked to IS26 insertions in the fljB promoter region of atypical monophasic variants of Salmonella enterica serovar Typhimurium.

molecular differences with a reference biphasic Salmonella Typhimurium strain could be observed. Next-generation sequencing of one representative isolate affected in the fljB promoter region revealed a 26-kb ...

False-positive results in metagenomic virus discovery: a strong case for follow-up diagnosis.

RNA, Viral specimen handling Viruses Abstract: A viral metagenomic approach using virion enrichment, random amplification and next-generation sequencing was used to investigate an undiagnosed cluster of ...

The origin of biased sequence depth in sequence-independent nucleic acid amplification and optimization for efficient massive parallel sequencing.

a 3’ random part used to prime complementary DNA synthesis and a 5’ defined tag sequence for subsequent amplification. Recently, this amplification method was combined with next generation sequencing to ...

What's in a strain? Viral metagenomics identifies genetic variation and contaminating circoviruses in laboratory isolates of pigeon paramyxovirus type 1.

used next generation sequencing on random amplified viral nucleic acids to determine the genome sequence of 11 pigeon paramyxovirus type 1 (PPMV-1) isolates from Belgium (period 1998-2011). The PPMV-1 ...

Phylogeographic analysis of avian influenza viruses isolated from Charadriiformes in Belgium confirms intercontinental reassortment in gulls.

random amplification and next-generation sequencing. The gene segments of these viruses segregated into three phylogeographic lineage types: (1) segments circulating in waterfowl in Eurasia with sporadic ...

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