Zoekresultaten - 45 results

Very Long Persistence of Botulinum Toxin B in a Patient’s Serum

immunocompromised adults) botulism. Case Report: A case of human botulism type B due to the consumption of an imported dried ham is reported. Laboratory results confirmed the diagnosis using the mouse bioassay and the qPCR ...

A novel genotoxin-specific qPCR array based on the metabolically competent human HepaRG™ cell line as a rapid and reliable tool for improved in vitro hazard assessment

Keersmaecker; Rogiers, Vera; Tatyana Y. Doktorova Source: Archives of Toxicology (2018) Keywords: genotoxicity HepaRG In vitro screening mutagenicity qPCR array Toxicogenomics Abstract: Although the value of the ...

Complete coding sequence of a novel picorna-like virus in a blackbird infected with Usutu virus.

consists of at least 9,228 nt and contains two open reading frames (ORF s) encoding the nonstructural polyprotein (2235 amino acids) and structural polyprotein (769 amino acids). Two TaqMan RT-qPCR assays ...

Nanopore sequencing technology: a new route for the fast detection of unauthorized GMO.

region. This DNA walking is performed on transgenic elements, commonly found in GMO, that were earlier detected by real-time PCR (qPCR) screening. Previously, we have demonstrated the ability of this ...

Development and validation of an integrated DNA walking strategy to detect GMO expressing cry genes

Characterization detection DNA walking GMO identification qPCR Abstract: Background Recently, an integrated DNA walking strategy has been proposed to prove the presence of GMO via the characterisation of sequences ...

Detection and discrimination of five E. coli pathotypes using a combinatory SYBR® Green qPCR screening system

five Escherichia coli pathotypes, based on a combination of 13 SYBR ® Green qPCR, has been developed, i.e., combinatory SYBR ® Green qPCR screening system for pathogenic E. coli (CoSYPS Path E. coli). It ...

Pertussis diagnosis in Belgium: results of the National Reference Centre for Bordetella anno 2015.

Young adult Abstract: In 2015, the Belgian National Reference Centre for Bordetella analyzed 4110 respiratory samples by qPCR and 4877 serum samples by serology. Whereas about 50% of respiratory samples ...

Laboratory validation of two real-time RT-PCR methods with 5'-tailed primers for an enhanced detection of foot-and-mouth disease virus.

and Specificity Sheep Swine Abstract: The 3D and 5UTR real-time RT- PCR assays (RT-qPCR) from Callahan et al. (2002) and Reid et al. (2002) are commonly used reference methods for the detection of ...

Discrimination of three genetically close Aspergillus species by using high resolution melting analysis applied to indoor air as case study.

discriminate between species which are genetically close. A solution to this could be the use of a post-qPCR high resolution melting (HRM) analysis, which would allow the discrimination of these species based on ...

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