Zoekresultaten - 32 results
A shotgun metagenomics approach to detect and characterize unauthorized genetically modified microorganisms in microbial fermentation products
fermentation products on the market is prohibited by European regulations. GMM s are currently screened for through qPCR assays targeting AMR genes and vectors, and then confirmed by targeting known specific GM ...
Discrimination of three genetically close Aspergillus species by using high resolution melting analysis applied to indoor air as case study.
discriminate between species which are genetically close. A solution to this could be the use of a post-qPCR high resolution melting (HRM) analysis, which would allow the discrimination of these species based on ...
How can we better detect unauthorized GMO in the food and feed chain
6, Number 517 (2017) Keywords: detection DNA walking Monitoring new workflow next-generation-sequencing qPCR unauthorized GMO Abstract: Current GMO detection systems have limited abilities to detect ...
An integrated strategy combining DNA walking and NGS to detect GMO
Volume 232, Number 358 (2017) Keywords: detection DNA walking GMO Next-generation sequencing qPCR Abstract: Recently, we developed a DNA walking system for the detection and characterization of a broad ...
Biotech Rice: Current developments and future detection challenges in food and feed chain
choice, regulatory frameworks were established in many countries around the world, such as in Europe. Their implementations, including detection systems usually based on qPCR, are becoming complex and ...
Current and new approaches in GMO detection: challenges and solutions
https://www.sciensano.be/nl/biblio/current-and-new-approaches-gmo-detection-challenges-and-solutions
DNA, have been developed to implement these legislations. Due to its numerous advantages, the quantitative PCR (qPCR) is the method of choice for the enforcement laboratories in GMO routine analysis. ...
Development and performance assessment of a qualitative SYBR® green real-time PCR assay for the detection of Aspergillus versicolor in indoor air.
Subsequently, the performance of this quantitative polymerase chain reaction (qPCR) method was assessed using specific criteria, including an evaluation of the selectivity, PCR efficiency, dynamic range, and ...
Integrated DNA walking system to characterize a broad spectrum of GMOs in food/feed matrices.
with qPCR screening, usually used in GMO routine analysis, as well as being able to detect, characterize and identify a broad spectrum of GMO s in food/feed matrices, two bidirectional DNA walking ...
Statistical framework for detection of Genetically Modified Organisms based on Next Generation Sequencing
number and diversity of genetically modified (GM) crops has significantly increased, their analysis based on real-time PCR (qPCR) methods is becoming increasingly complex and laborious. While several ...
New trait-specific qualitative SYBR®Green qPCR methods to expand the panel of GMO screening methods used in the CoSYPS
European Food Research and Technology, Volume 241, Issue 2 (2015) Keywords: New qualitative trait-specific SYBR®Green qPCR methods to expand the panel of GMO screening methods used in the CoSYPS Abstract: ...