Zoekresultaten - 10 results
Risk Factors Associated with Severe RSV Infection in Infants: What Is the Role of Viral Co-Infections?
for a panel of 16 different respiratory viruses with multiplex RT-qPCR. Disease severity was assessed with traditional clinical parameters and scoring systems. A total of 120 patients were included, of ...
Evaluating Diagnostic Accuracy of Saliva Sampling Methods for Severe Acute Respiratory Syndrome Coronavirus 2 Reveals Differential Sensitivity and Association with Viral Load.
>2500 individuals. All samples were tested by RT-qPCR, detecting RNA of SARS-CoV-2. The test sensitivity was compared on the two saliva collections with the nasopharyngeal specimen for all subjects and ...
QuilA-Adjuvanted T. gondii Lysate Antigens Trigger Robust Antibody and IFNγ+ T Cell Responses in Pigs Leading to Reduction in Parasite DNA in Tissues Upon Challenge InfectionImage
Immunology, Volume 10 (2019) Keywords: magnetic capture-qPCR Pigs QuilA TLA vaccine Toxoplasma gondii Abstract: Toxoplasma gondii is an intracellular parasite of all mammals and birds, responsible for ...
A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses
Dominique Rousset; Steven Van Gucht; Nancy Roosens Source: International Journal of Infectious Diseases, Volume 92 (2020) Keywords: Chikungunya virus discrimination identification multiplex RT-qPCR Zika virus ...
A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses.
Publication Type: Peer reviewed scientific article Authors: Sylvia Broeders; Linda Garlant; Marie-Alice Fraiture; Els Vandermassen; Vanessa Suin; Jessica Vanhomwegen; Myrielle Dupont-Rouzeyrol; Dominique Rousset; Steven Van Gucht; Nancy Roosens Source: In ...
Application of whole genome data for in silico evaluation of primers and probes routinely employed for the detection of viral species by RT-qPCR using dengue virus as a case study.
real-time polymerase chain reaction (RT-qPCR) directed against genomic regions conserved between different isolates. Genetic variation can however result in mismatches of primers and probes with their ...
Very Long Persistence of Botulinum Toxin B in a Patient’s Serum
immunocompromised adults) botulism. Case Report: A case of human botulism type B due to the consumption of an imported dried ham is reported. Laboratory results confirmed the diagnosis using the mouse bioassay and the qPCR ...
Evaluation of SNPs based on DNA extracted from urine as part of the development and use of a kit of non-invasive biomarkers to monitor respiratory health of young children
Publication Type: Scientific poster, presentation or proceeding Authors: S. Nauwelaerts; Koen De Cremer; Bernard, Alfred; Nancy Roosens; Sigrid C.J. De Keersmaecker Source: qPCR dPCR & NGS 2017 ...
A multifaceted study of Pseudomonas aeruginosa shutdown by virulent podovirus LUZ19
quantitative PCR (qPCR), microarray, RNA-seq, and two-dimensional gel electrophoresis (2D- GE), to obtain a global overview of alterations in DNA, RNA, and protein content in Pseudomonas aeruginosa PAO1 cells ...
Prevalence and incidence of anti-SARS-CoV-2 antibodies among healthcare workers in Belgian hospitals before vaccination: a prospective cohort study.
antibodies were measured using a virus neutralisation test. Real-time reverse transcription PCR (RT-qPCR) was performed to detect SARS-CoV-2 on nasopharyngeal swabs. Participant characteristics and the ...