Zoekresultaten - 122 results
Development of a molecular detection platform for GMO detection in food based on "combinatory qPCR" technology
Publication Type: Sci. report, recommendat°, guidance doc., directive, monograph Authors: Nancy Roosens; E. Barbau-Piednoir; A. Lievens; G.E. Mbongolo-Mbella; D. Van Geel; Els Vandermassen; Amaya Leunda; Sylvia Broeders; Myriam Sneyers; Marc Van den Bulck ...
Simultaneous detection of bluetongue virus RNA, internal control GAPDH mRNA, and external control synthetic RNA by multiplex real-time PCR.
insect-borne disease of domestic and wild ruminants that requires strict monitoring by sensitive, reproducible and robust methods. Real-time reverse transcription polymerase chain reaction (RT-qPCR) analysis has ...
A combinatory qPCR screening system to detect food-borne pathogens
Department of Food Safety and Food Quality,UGent, NA (2010) Keywords: a combinatory conference food Food Microbiology microbiology ON pathogen qPCR SCREENING System Health Topics: Food consumption and food ...
A proposed validation method for automated nucleic acid extraction and RT-qPCR analysis: an example using Bluetongue virus.
Transcriptase Polymerase Chain Reaction Validation Studies as Topic Abstract: This study proposes a validation strategy for an automated extraction procedure, followed by RT-qPCR analysis. To avoid false-negative ...
A theoretical introduction to "combinatory SYBRGreen qPCR screening", a matrix-based approach for the detection of materials derived from genetically modified plants.
identification, and often quantification of the genetically modified organisms (GMO) present in a sample. “Combinatory qPCR SYBRG reen screening” (CoSYPS) is a matrix-based approach for determining the presence of ...
Emergence of bluetongue serotypes in Europe, part 1: description and validation of four real-time RT-PCR assays for the serotyping of bluetongue viruses BTV-1, BTV-6, BTV-8 and BTV-11.
the currently circulating BTV serotypes in field samples. In the present study, four serotype-specific real-time RT- PCR assays (RT-qPCR) are described for the detection of the BTV-1, BTV-6, BTV-8 and ...
Emergence of bluetongue serotypes in Europe, part 2: the occurrence of a BTV-11 strain in Belgium.
died soon after delivery, was shown to be positive for bluetongue virus (BTV)- RNA using a group-specific real-time RT- PCR (RT-qPCR). In-house serotype-specific RT-qPCR assays for bluetongue virus ...
The use of q-PCR for GMO detection in an NRL laboratory
GMO detection Laboratories qPCR use Workshop Health Topics: Food consumption and food safety Consommation et sécurité alimentaires Voedselconsumptie en –veiligheid Service: Activités transversales en ...
Towards an integrated Real-Time Q-PCR platform for GMO detection at low level presence
Co-extra International Congress, NA (2009) Keywords: an at Co-Extra Congresses detection GMO GMO detection International LEVEL low level qPCR Health Topics: Food consumption and food safety Consommation et ...
Bluetongue virus detection by real-time RT-PCR in Culicoides captured during the 2006 epizootic in Belgium and development of an internal control.
reverse transcription PCR (RT-qPCR) targeting bluetongue virus (BTV) segment 5. To ensure the RNA integrity of the samples, all pools were also tested in a second RT-qPCR targeting Culicoides 18S rRNA, ...