Zoekresultaten - 122 results
Laboratory validation of two real-time RT-PCR methods with 5'-tailed primers for an enhanced detection of foot-and-mouth disease virus.
and Specificity Sheep Swine Abstract: The 3D and 5UTR real-time RT- PCR assays (RT-qPCR) from Callahan et al. (2002) and Reid et al. (2002) are commonly used reference methods for the detection of ...
Discrimination of three genetically close Aspergillus species by using high resolution melting analysis applied to indoor air as case study.
discriminate between species which are genetically close. A solution to this could be the use of a post-qPCR high resolution melting (HRM) analysis, which would allow the discrimination of these species based on ...
Evaluation of SNPs based on DNA extracted from urine as part of the development and use of a kit of non-invasive biomarkers to monitor respiratory health of young children
Publication Type: Scientific poster, presentation or proceeding Authors: S. Nauwelaerts; Koen De Cremer; Bernard, Alfred; Nancy Roosens; Sigrid C.J. De Keersmaecker Source: qPCR dPCR & NGS 2017 ...
How can we better detect unauthorized GMO in the food and feed chain
6, Number 517 (2017) Keywords: detection DNA walking Monitoring new workflow next-generation-sequencing qPCR unauthorized GMO Abstract: Current GMO detection systems have limited abilities to detect ...
An integrated strategy combining DNA walking and NGS to detect GMO
Volume 232, Number 358 (2017) Keywords: detection DNA walking GMO Next-generation sequencing qPCR Abstract: Recently, we developed a DNA walking system for the detection and characterization of a broad ...
Development of molecular tools for rapid detection and quantification of indoor airborne molds to assess their impact on public health
a specific ITS-based SYBR ®green real-time PCR (qPCR) assay for Aspergillus versicolor, a species frequently observed in indoor air and known to be allergenic. Additionally, an ITS-based qPCR assay was ...
Development of molecular tools for rapid detection of indoor airborne molds to assess their impact on public health
Development Fungi Impact Indoor Indoor air Luminex Molecular molecular detection NGS ON public qPCR Health Topics: Health and environment Santé et environnement Gezondheid en milieu Service: Activités ...
A Versatile Panel of Reference Gene Assays for the Measurement of Chicken mRNA by Quantitative PCR.
therefore attached special importance to testing each qPCR assay for absence of signal using DNA template. The assays and analyses developed here provide a useful resource for selecting reference genes for ...
A Tale of Tails: Dissecting the Enhancing Effect of Tailed Primers in Real-Time PCR.
conducted a detailed in vitro and in silico analysis of the enhancing effect of primer tailing on 2 well-established foot-and-mouth disease virus (FMDV) RT-qPCR assays using an FMDV reference panel. Tailing ...
Biotech Rice: Current developments and future detection challenges in food and feed chain
choice, regulatory frameworks were established in many countries around the world, such as in Europe. Their implementations, including detection systems usually based on qPCR, are becoming complex and ...