Zoekresultaten - 16 results
What's in a strain? Viral metagenomics identifies genetic variation and contaminating circoviruses in laboratory isolates of pigeon paramyxovirus type 1.
viruses or sequence variants from the original sample may persist in the stocks without being identified by the routine virus-specific diagnostic tools. The exact role of PiCV in pigeon disease — in ...
Further evidence for the widespread co-circulation of lineages 4b and 7 velogenic Newcastle disease viruses in rural Nigeria.
routine real-time reverse transcriptase-polymerase chain reaction detection) were isolated in six states during the two-year period. The documented genetic variants occurred over a large geographic area, ...
Methodologies for Salmonella enterica subsp. enterica subtyping: gold standards and alternatives.
routinely performed by serotyping, a method in which surface antigens are identified based on agglutination reactions with specific antibodies. The serotyping scheme, which is continuously updated as new ...
Classical swine fever virus detection: results of a real-time reverse transcription polymerase chain reaction ring trial conducted in the framework of the European network of excellence for epizootic disease diagnosis and control.
reaction (real-time RT- PCR) ring trial for the detection of Classical swine fever virus (CSFV) genomic RNA undertaken by 10 European laboratories. All laboratories were asked to use their routine in-house ...
Diagnostic performance and application of two commercial cell viability assays in foot-and-mouth disease research.
plates. The CTB (abs) assay is now used routinely in the Belgian FMD reference laboratory for serological testing and high-throughput antiviral compound screening. Health Topics: Animal health Category: ...
Influence of the incubation temperature and the batch components on the sensitivity of an enzyme-linked immunosorbent assay to detect Aujeszky's disease virus glycoprotein E (gE).
immunosorbent assay (ELISA) for Aujeszky’s disease virus (ADV) were used, and the assays were performed within an ISO / IEC 17025 accredited quality control system, certain routine runs of the ADV ELISA were not ...
Simultaneous detection of bluetongue virus RNA, internal control GAPDH mRNA, and external control synthetic RNA by multiplex real-time PCR.
become the method of choice for routine viral diagnosis. As false-negative test results can have serious implications; an internal/external control system should be incorporated in each analysis to detect ...
Validation of two real-time RT-PCR methods for foot-and-mouth disease diagnosis: RNA-extraction, matrix effect, uncertainty of measurement and precision.
Specificity Abstract: Real-time reverse transcription polymerase chain reaction (rRT- PCR) assays are being used routinely for diagnosing foot-and-mouth disease virus (FMDV). Although most laboratories ...
Comparison of classical serotyping and PremiTest assay for routine identification of common Salmonella enterica serovars.
routine identification of Salmonella strains of animal origin. A blind analysis of 754 strains was conducted in parallel by classical serotyping and the PremiTest assay. Full results were available for 685 ...
Effect of pooling and multiplexing on the detection of bluetongue virus RNA by real-time RT-PCR.
(RT-qPCR) was used routinely for laboratory diagnosis during the 2006/2007 bluetongue virus (BTV) serotype 8 epidemic. In the present study the impact of pooling and multiplexing strategies on RT-qPCR are ...