Zoekresultaten - 137 results

The GEN-ERA toolbox: unified and reproducible workflows for research in microbial genomics

need to implement new standards in species delineation, including whole-genome sequencing and phylogenomics. In this context, the genomic needs of the Belgian Coordinated Collections of Microorganisms ...

Outbreak of Corynebacterium diphtheriae among asylum seekers in Belgium in 2022: operational challenges and lessons learnt.

Hoorelbeke, Bart; Piérard, Denis; Laura Cornelissen Source: Euro Surveill, Volume 28, Issue 44 (2023) Keywords: Belgium Corynebacterium diphtheriae Diphtheria Disease Outbreaks Humans Male Multilocus Sequence ...

Targeted Chromosomal Barcoding Establishes Direct Genotype-Phenotype Associations for Antibiotic Resistance in Mycobacterium abscessus.

bedaquiline-resistant M. abscessus colonies transformed with both substrates, we obtained equivalent numbers of recombinants. These resistant colonies were analyzed by allele-specific PCR and Sanger sequencing, and we ...

Development of a Taxon-Specific Real-Time Polymerase Chain Reaction Method to Detect Trichoderma reesei Contaminations in Fermentation Products

food industry to produce fermentation products, a novel real-time PCR method specific to this fungal species was developed and validated in this study to reinforce the GMM detection strategy. Designed to ...

Targeted High-Throughput Sequencing Enables the Detection of Single Nucleotide Variations in CRISPR/Cas9 Gene-Edited Organisms

experimental-based evidence indicating that the proposed targeted high-throughput sequencing approach may constitute, in the future, a specific and sensitive tool to support the safety and traceability of the ...

Population Analysis of O26 Shiga Toxin-Producing Causing Hemolytic Uremic Syndrome in Italy, 1989-2020, Through Whole Genome Sequencing.

Hemolytic-Uremic Syndrome Humans ITALY Multilocus Sequence Typing Shiga-Toxigenic Escherichia coli whole genome sequencing Abstract: Shiga toxin-producing (STEC) belonging to the O26 serogroup represent an important ...

Development of a taxon-specific real-time PCR method targeting the Bacillus subtilis group to strengthen the control of genetically modified bacteria in fermentation products

taxon-specific method allows the strengthening of the set of screening markers that are targeting key sequences that are frequently found in GM bacteria (AMR genes and shuttle vector), reinforcing control over the ...

Shotgun metagenomics as a ONE Health tool for better protecting human health

identify all biological material in a sample without a priori knowing what to look for, could transform the way specific key public health questions can be addressed, in a ONE Health context. Metagenomics is ...

Development of a data-intensive centralized system for surveillance and outbreak investigation of bacterial pathogens using whole-genome sequencing

Publication Type: Dissertation Authors: Bert Bogaerts Source: Ghent University, Gh, p.250 (2021) Keywords: bioinformatics public health VALIDATION whole-genome sequencing Abstract: Whole genome ...

Strategy and Performance Evaluation of Low-Frequency Variant Calling for SARS-CoV-2 Using Targeted Deep Illumina Sequencing

available at https://galaxy.sciensano.be, and specific recommendations for minimum sequencing coverages to detect clade-defining mutations at certain allelic frequencies. This approach will be useful to ...

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