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Home > Biblio > Molecular subtyping of Salmonella Typhimurium with multiplex oligonucleotide ligation-PCR (MOL-PCR)

Molecular subtyping of Salmonella Typhimurium with multiplex oligonucleotide ligation-PCR (MOL-PCR) [1]

Scientific book or chapter

SCIENSANO

Authors

Wuyts,V. [2]; Wesley Mattheus [3]; Nancy Roosens [4]; Marchal,K. [5]; Sophie Bertrand [6]; Sigrid C.J. De Keersmaecker [7]

Keywords

  1. a [8]
  2. analysi [9]
  3. analysis [10]
  4. AS [11]
  5. bacteria [12]
  6. bead suspension array [13]
  7. Biology [14]
  8. Control [15]
  9. data [16]
  10. detection [17]
  11. Diagnosis [18]
  12. disease [19]
  13. Diseases [20]
  14. Dna [21]
  15. Genetic [22]
  16. Gödel Prime Product [23]
  17. high-throughput assay [24]
  18. IS [25]
  19. Isolation [26]
  20. IT [27]
  21. Luminex [28]
  22. lysis [29]
  23. Marker [30]
  24. Markers [31]
  25. method [32]
  26. methods [33]
  27. MOL-PCR [34]
  28. Molecular [35]
  29. Molecular biology [36]
  30. molecular markers [37]
  31. molecular subtyping [38]
  32. Multiplex assay [39]
  33. ON [40]
  34. pathogen [41]
  35. PCR [42]
  36. PRODUCTS [43]
  37. protocol [44]
  38. S [45]
  39. Salmonella [46]
  40. Salmonella enterica [47]
  41. Salmonella typhimurium [48]
  42. Subtyping [49]
  43. Technique [50]
  44. Type [51]
  45. Viruses [52]

Abstract:

A multiplex oligonucleotide ligation-PCR (MOL-PCR) assay is a valuable high-throughput technique for the detection of bacteria and viruses, for characterization of pathogens and for diagnosis of genetic diseases, as it allows to combine different types of molecular markers in a high-throughput multiplex assay. A MOL-PCR assay starts with a multiplex oligonucleotide ligation reaction for detection of the molecular marker, followed by a singleplex PCR for signal amplification and analysis of the MOL-PCR products on a Luminex platform. This last step occurs through a liquid bead suspension arr…
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Source URL:https://www.sciensano.be/en/biblio/molecular-subtyping-salmonella-typhimurium-multiplex-oligonucleotide-ligation-pcr-mol-pcr

Links
[1] https://www.sciensano.be/en/biblio/molecular-subtyping-salmonella-typhimurium-multiplex-oligonucleotide-ligation-pcr-mol-pcr [2] https://www.sciensano.be/en/biblio?f%5Bauthor%5D=1041&f%5Bsearch%5D=Wuyts%2CV. [3] https://www.sciensano.be/en/people/wesley-mattheus/biblio [4] https://www.sciensano.be/en/people/nancy-roosens/biblio [5] https://www.sciensano.be/en/biblio?f%5Bauthor%5D=1044&f%5Bsearch%5D=Marchal%2CK. [6] https://www.sciensano.be/en/people/sophie-bertrand/biblio [7] https://www.sciensano.be/en/people/sigrid-de-keersmaecker/biblio [8] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=1029&f%5Bsearch%5D=a [9] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=1806&f%5Bsearch%5D=analysi [10] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=1506&f%5Bsearch%5D=analysis [11] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=846&f%5Bsearch%5D=AS [12] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=1260&f%5Bsearch%5D=bacteria [13] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=25215&f%5Bsearch%5D=bead%20suspension%20array [14] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=1047&f%5Bsearch%5D=Biology [15] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=1332&f%5Bsearch%5D=Control [16] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=1335&f%5Bsearch%5D=data [17] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=540&f%5Bsearch%5D=detection [18] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=1338&f%5Bsearch%5D=Diagnosis [19] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=1266&f%5Bsearch%5D=disease [20] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=1341&f%5Bsearch%5D=Diseases [21] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=1149&f%5Bsearch%5D=Dna [22] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=2430&f%5Bsearch%5D=Genetic [23] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=25218&f%5Bsearch%5D=G%C3%B6del%20Prime%20Product [24] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=25221&f%5Bsearch%5D=high-throughput%20assay [25] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=525&f%5Bsearch%5D=IS [26] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=3867&f%5Bsearch%5D=Isolation [27] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=1200&f%5Bsearch%5D=IT [28] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=17556&f%5Bsearch%5D=Luminex [29] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=2058&f%5Bsearch%5D=lysis [30] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=2451&f%5Bsearch%5D=Marker [31] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=2877&f%5Bsearch%5D=Markers [32] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=1134&f%5Bsearch%5D=method [33] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=1359&f%5Bsearch%5D=methods [34] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=24972&f%5Bsearch%5D=MOL-PCR [35] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=1059&f%5Bsearch%5D=Molecular [36] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=1053&f%5Bsearch%5D=Molecular%20biology [37] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=3279&f%5Bsearch%5D=molecular%20markers [38] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=25224&f%5Bsearch%5D=molecular%20subtyping [39] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=1014&f%5Bsearch%5D=Multiplex%20assay [40] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=912&f%5Bsearch%5D=ON [41] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=972&f%5Bsearch%5D=pathogen [42] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=396&f%5Bsearch%5D=PCR [43] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=915&f%5Bsearch%5D=PRODUCTS [44] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=918&f%5Bsearch%5D=protocol [45] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=2475&f%5Bsearch%5D=S [46] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=2151&f%5Bsearch%5D=Salmonella [47] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=2478&f%5Bsearch%5D=Salmonella%20enterica [48] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=5217&f%5Bsearch%5D=Salmonella%20typhimurium [49] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=4782&f%5Bsearch%5D=Subtyping [50] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=561&f%5Bsearch%5D=Technique [51] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=1026&f%5Bsearch%5D=Type [52] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=690&f%5Bsearch%5D=Viruses