Prediction of Susceptibility to First-Line Tuberculosis Drugs by DNA Sequencing
Health and disease monitoring
Peer reviewed scientific article
English
SCIENSANO
Authors
The CRyPTIC Consortium and the 100000 Genomes Project [1]; Allix-Béguec, Caroline [2]; Irena Arandjelovic [3]; Lijun Bi [4]; Patrick Beckert [5]; Maryline Bonnet [6]; Phelim Bradley [7]; Andrea Cabibbe [8]; Irving Cancino-Muñoz [9]; Mark J. Caulfield [10]; Angkana Chaiprasert [11]; Daniela Cirillo [12]; David Clifton [13]; Iñaki Comas [14]; Derrick W Crook [15]; Maria R De Filippo [16]; Han De Neeling [17]; Roland Diel [18]; Francis A. Drobniewski [19]; Kiatichai Faksri [20]; Maha R. Farhat [21]; Joy Fleming [22]; Philip Fowler [23]; Tom A. Fowler [24]; Gao, Qian [25]; Jennifer Gardy [26]; Deborah Gascoyne-Binzi [27]; Ana-Luiza Gibertoni-Cruz [28]; Ana Gil-Brusola [29]; Tanya Golubchik [30]; Ximena Gonzalo [31]; Louis Grandjean [32]; Guangxue, He [33]; Jennifer L. Guthrie [34]; Sarah Hoosdally [35]; Martin Hunt [36]; Zamin Iqbal [37]; Nazir Ismail [38]; James Johnston [39]; Faisal Khanzada [40]; Chiea Khor [41]; Thomas A. Kohl [42]; Clare Kong [43]; Sam Lipworth [44]; Liu, Qingyun [45]; Gugu Maphalala [46]; Elena Martinez [47]; Vanessa Mathys [48]; Matthias Merker [49]; Paolo Miotto [50]; Mistry, Nerges [51]; David A.J. Moore [52]; Megan Murray [53]; Stefan Niemann [54]; Rick T.-H. Ong [55]; Tim E.A. Peto [56]; James E. Posey [57]; Prammananan, Therdsak [58]; Alexander Pym [59]; Camilla Rodrigues [60]; Mabel Rodrigues [61]; Timothy Rodwell [62]; Gian M. Rossolini [63]; Elisabeth Sánchez Padilla [64]; Marco Schito [65]; Xin Shen [66]; Jay Shendure [67]; Vitali Sintchenko [68]; Alex Sloutsky [69]; Grace E. Smith [70]; Matthew Snyder [71]; Karine Soetaert [72]; Angela M. Starks [73]; Supply, Philip [74]; Prapat Suriyapol [75]; Sabira Tahseen [76]; Patrick Tang [77]; Teo, Yik-Ying [78]; Thuong N.T. Thuong [79]; Guy Thwaites [80]; Tortoli, Enrico [81]; Shaheed V. Omar [82]; Dick van Soolingen [83]; Sarah A. Walker [84]; Timothy M. Walker [85]; Mark Wilcox [86]; Daniel J. Wilson [87]; David Wyllie [88]; Yang, Yang [89]; Hongtai Zhang [90]; Zhao, Yanlin [91]; Zhu, Baoli [92]Keywords
Abstract:
BACKGROUND: The World Health Organization recommends drug-susceptibility testing of Mycobacterium tuberculosis complex for all patients with tuberculosis to guide treatment decisions and improve outcomes. Whether DNA sequencing can be used to accurately predict profiles of susceptibility to first-line antituberculosis drugs has not been clear.METHODS: We obtained whole-genome sequences and associated phenotypes of resistance or susceptibility to the first-line antituberculosis drugs isoniazid, rifampin, ethambutol, and pyrazinamide for isolates from 16 countries across six continents. For e…