Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification.
Ziekten en gezondheid in kaart brengen
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David Rodriguez-Temporal [3]; Fernando Alcaide [4]; Ivana Mareković [5]; James Anthony O'Connor [6]; Rebecca Gorton [7]; Jakko van Ingen [8]; An Van den Bossche [9]; Genevieve Héry-Arnaud [10]; Clémence Beauruelle [11]; Dorothea Orth-Höller [12]; Juan-José Palacios-Gutiérrez [13]; Griselda Tudó [14]; Germán Bou [15]; Pieter-Jan Ceyssens [16]; Montserrat Garrigó [17]; Julià González-Martin [18]; Gilbert Greub [19]; Jaroslav Hrabak [20]; André Ingebretsen [21]; Maria Concepción Mediavilla-Gradolph [22]; Marina Oviaño [23]; Begoña Palop [24]; Arthur B Pranada [25]; Lidia Quiroga [26]; Maria Jesús Ruiz-Serrano [27]; Belén Rodriguez-Sanchez [28]Trefwoorden
Samenvatting:
The ability of MALDI-TOF for the identification of nontuberculous mycobacteria (NTM) has improved recently thanks to updated databases and optimized protein extraction procedures. Few multicentre studies on the reproducibility of MALDI-TOF have been performed so far, none on mycobacteria. The aim of this study was to evaluate the reproducibility of MALDI-TOF for the identification of NTM in 15 laboratories in 9 European countries. A total of 98 NTM clinical isolates were grown on Löwenstein-Jensen. Biomass was collected in tubes with water and ethanol, anonymized and sent out to the 15 part…