Transmission electron microscopy analysis request [1]
Sciensano conducts various electron microscopic analyses using transmission electron microscopy (TEM). These include the identification of nanomaterials according to the EC definition (2011/ 696/ EU), the detailed characterization of nanomaterials as part of risk assessments, classification and toxicological evaluation and characterization of virus-like particles.
TEM analyses provided for identification and characterisation of nanomaterials
- sample preparation (dispersion) of powder samples:
- NANoREG protocol
- Guiot & Spalla protocol
- Tailor-made sample preparation
- PTA analysis of nanomaterials in a stable dispersion
- DLS analysis of nanomaterials in a stable dispersion
- Zeto potential measurement of nanomaterials in a stable dispersion
- Preparation of TEM specimen and descriptive TEM characterisation (assuming stable dispersion)
- Advanced TEM characterisation
- Quantitative
- TEM characterisation of aggregates / agglomerates (image recording and analysis)
- TEM characterisation of primary particles (image recording and analysis)
- Diffraction
- Crystal structure (powder electron diffraction)
- Quantitative
TEM analyses provided for characterisation of virus-like particles (VLPs)
Human and veterinary vaccines
- visualization with TEM of the negatively stained VLPs in dispersion and of potential contaminants in representative and selected micrographies
- description of the morphology of the observed VLPs
- rough estimate of the number of VLPs based on the number of VLPs per surface of the EM grid
Jan Mast [2] for further information and prices or if you are interested in similar analyses that are not listed here. We may well have a solution for you.