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Strategy and Performance Evaluation of Low-Frequency Variant Calling for SARS-CoV-2 Using Targeted Deep Illumina Sequencing
approach and Illumina sequencing. As anticipated, higher variation and lower sensitivity were observed at lower coverages and allelic frequencies. We found that detection of all low-frequency variants at an ...
Lentiviral vector induces high-quality memory T cells via dendritic cells transduction.
lentiviral vector provided enhance therapeutic anti-tumor protection than Ad5. In conclusion, coupling lentiviral vector with β2-microglobulin promoter represents a promising approach to produce long-lasting, ...
Clinical Origin and Species Distribution of spp. Isolates Identified by Molecular Sequencing and Mass Spectrometry: A European Multicenter Hospital Prospective Study.
identify isolates at the species level. The identification was performed by PCR-sequencing and phylogenic-tree approach. Both methods are used as gold standard for the evaluation of mass spectrometry. ...
The taxonomy of the Trichophyton rubrum complex: a phylogenomic approach
Publication Type: Peer reviewed scientific article Authors: Luc Cornet; Elizabet D'hooge; Nicolas Magain; Dirk Stubbe; Ann Packeu; Denis Baurain; Pierre Becker Source: Microbial Genomics, Volume 7, Issue 11 (2021) Keywords: Dermatophytes Fungi Genomi ...
Position paper on the use of an “estimated acceptable concentration” (EAC) as basis for a control policy's action level for carcinogens unintentionally present in food
absence of legal maximum limits, the competent authority may act if high contamination levels that endanger public health are identified. A pragmatic approach to identify whether a value is potentially too ...
Update of the risk assessment of nickel in food and drinking water
lowest‐observed‐adverse‐effect‐level of 4.3 μg Ni/kg bw was selected as the reference point. The margin of exposure (MOE) approach was applied and an MOE of 30 or higher was considered as being indicative of a low health concern. The ...
Risk assessment of glycoalkaloids in feed and food, in particular in potatoes and potato‐derived products
studies. Occurrence data were available only for α‐solanine and α‐chaconine, mostly for potatoes. The acute dietary exposure to potato GA s was estimated using a probabilistic approach and applying ...
Use of a Whole Genome Sequencing-based approach for Mycobacterium tuberculosis surveillance in Europe in 2017-2019: an ECDC pilot study.
molecular typing and characterisation of complex (MTBC) strains. We evaluated the systematic use of a WGS-based approach for MTBC surveillance involving all European Union/European Economic Area (EU / EEA) ...
Risk assessment of ochratoxin A in food
Keywords: dietary exposure assessment hazard characterisation margin of exposure approach Ochratoxin risk characterisation Abstract: The European Commission asked EFSA to update their 2006 opinion on ...
ADVANCE database characterisation and fit for purpose assessment for multi-country studies on the coverage, benefits and risks of pertussis vaccinations.
approach is ready to be used for other vaccines/events to create readiness for participation in other vaccine related studies. Health Topics: Health and disease monitoring Surveillance de la santé et des ...