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The most likely time and place of introduction of BTV8 into Belgian ruminants.
been performed to enable determination of the initial spatiotemporal occurrence of this emerging disease in southern Belgium: investigations of the first recorded outbreaks near to the disease epicenter; ...
Aggregate size and architecture determine microbial activity balance for one-stage partial nitritation and anammox.
in partial nitritation/anammox systems to remove ammonium from wastewater. In this process, large granular microbial aggregates enhance the performance, but little is known about granulation so far. In ...
Emergence of bluetongue serotypes in Europe, part 1: description and validation of four real-time RT-PCR assays for the serotyping of bluetongue viruses BTV-1, BTV-6, BTV-8 and BTV-11.
variations were found to be low with a total coefficient of variation of 1-2% for clear positive samples and <10% for very weak positive samples. Finally, the performance of the described assays was ...
Expert judgement in a risk assessment model for Salmonella spp. in pork: the performance of different weighting schemes.
the uncertainty on the model input parameters. The performance of experts as probability assessors was measured by the experts’ ability to correctly and precisely provide estimates for a set of seed ...
Immunologic response of unvaccinated workers exposed to anthrax, Belgium.
https://www.sciensano.be/en/biblio/immunologic-response-unvaccinated-workers-exposed-anthrax-belgium
testing of workers in a factory that performed scouring of wool and goat hair. Of 66 workers, approximately 10% had circulating antibodies or T lymphocytes that reacted with anthrax protective antigen. ...
Validation of two real-time RT-PCR methods for foot-and-mouth disease diagnosis: RNA-extraction, matrix effect, uncertainty of measurement and precision.
not performed or reported generally. In this study, different RNA-extraction procedures were compared for two FMDV rRT- PCR s. The NucleoSpin columns available commercially combined high extraction ...
Epidemiological study of bovine norovirus infection by RT-PCR and a VLP-based antibody ELISA.
diarrhoeic calves screened by RT- PCR. Nucleotide sequencing performed on the polymerase region classified all the norovirus amplicons in the bovine norovirus genotype 2. Rather surprisingly, some rotavirus ...
Vaccination of birds other than chickens and turkeys against avian influenza.
of serological data. Whenever experimental challenge was performed in birds other than chickens and turkeys, vaccination using inactivated vaccines always protected against disease and mortality, ...
The major portal of entry of koi herpesvirus in Cyprinus carpio is the skin.
further demonstrate the role of the skin as the KHV portal of entry, we constructed an original system, nicknamed “U-tube,” to perform percutaneous infection restricted to the posterior part of the fish. ...
Technical note: Flow cytometric identification of bovine milk neutrophils and simultaneous quantification of their viability.
iodide was used to determine milk PMNL viability. Thirty milk samples were run in parallel to assess the repeatability of the immunoassay and 6 repeated measurements per sample were performed to assess the ...