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European interlaboratory comparison of Schmallenberg virus (SBV) real-time RT-PCR detection in experimental and field samples: The method of extraction is critical for SBV RNA detection in semen.
molecular detection methods used at European laboratories have not yet been assessed, to our knowledge. The performances of extraction and real-time reverse transcription polymerase chain reaction (RT-qPCR) ...
Detection and Isolation of Swine Influenza A Virus in Spiked Oral Fluid and Samples from Individually Housed, Experimentally Infected Pigs: Potential Role of Porcine Oral Fluid in Active Influenza A Virus Surveillance in Swine.
period in nasal swab samples. Therefore, more sensitive diagnostic methods to monitor circulating virus strains are requisite. METHODS: qRT- PCR and virus isolations were performed on oral fluid and nasal ...
Controlling of CSFV in European wild boar using oral vaccination: a review.
efficacious and palatable baits were developed for oral delivery in free ranging wild boars. The first field trials were performed in Germany in the 1990’s and allowed deploying oral baits at a large scale. The ...
Development and application of a non-target extraction method for the analysis of migrating compounds from plastic baby bottles by GC-MS.
selected a 50% (v/v) EtOH in water solution as a simulant for milk. After sterilization of the bottle during ten minutes with boiling water, three migrations were performed during 2h at 70 °C. The principal ...
Guidelines for optimisation of a multiplex oligonucleotide ligation-PCR for characterisation of microbial pathogens in a microsphere suspension array.
a singleplex PCR and analysis with microspheres on a Luminex device, several parameters are accessible for optimisation. Although MOL- PCR performance may be influenced by the markers used in the assay and the ...
Cereulide food toxin, beta cell function and diabetes: Facts and hypotheses.
death, both key traits in the pathophysiology of diabetes. In vivo studies of chronic or repeated low dose exposure to cereulide are currently lacking, but should be performed to further clarify the true ...
Physical Characterization of Nanomaterials in Dispersion by Transmission Electron Microscopy in a Regulatory Framework
of primary particle properties, and validation of the measurement results can be performed. The possibility to extract 3D information by fractal analysis of electron micrographs of aggregated ...
Impact of a trace element supplementation programme on health and performance of cross-breed (Bos indicus x Bos taurus) dairy cattle under tropical farming conditions: a double-blinded randomized field trial36964
electronic farms Field Group health i immune response Impact Increase intervention IS journal Laboratories milk n NUTRITION observed ON parameters performance plasma present programme protein Proteins Rabies ...
Salmonella enterica serotype enteritidis in French Polynesia, South Pacific, 2008-201336984
during 2008-2013. Molecular analysis of isolates by using clustered regularly interspaced short palindromic repeat polymorphisms and multilocus variable-number tandem-repeat analysis was performed. This ...
Development of a Stationary Phase Optimised Selectivity Liquid Chromatography based screening method for adulterations of food supplements for the treatment of pain.
the screening to be performed with diode array detection and is fully compatible with mass spectrometry. The method was validated for its selectivity following the guidelines as described for the ...