Search results - 413 results
Differentiation of the seven serotypes of foot-and-mouth disease virus by reverse transcriptase polymerase chain reaction.
transcriptase polymerase chain reaction (RT- PCR) using multiple primers was developed to detect and to differentiate the seven serotypes of foot-and-mouth disease virus (FMDV) simultaneously, quickly and ...
Rapid diagnosis of encephalomyocarditis virus infections in pigs using a reverse transcription-polymerase chain reaction.
the time-consuming seroneutralisation assay, currently used for the characterisation of EMCV, is described. An EMCV specific reverse transcription-polymerase chain reaction (RT- PCR), using primers ...
Epidemiological characteristics of an outbreak of classical swine fever in an area of high pig density.
only clinically healthy but some virologically positive pigs were serologically negative. Antigen detection was therefore critical for early disease detection. Serology was nevertheless useful to ...
Quantitative image analysis as an alternative to chemical analysis for follow-up of liver biopsies from a toucan with hemochromatosis. A technique with potential value for the follow-up of hemochromatosis in humans.
toucan treated with deferoxaminemesilate in combination with a low-iron diet. STUDY DESIGN: Two different image analysis systems were used, one developed for working with black-and-white pictures and ...
Cellular pathways involved in the ex vivo expression of bovine leukemia virus.
mechanism, we employed a general approach using chemicals that interfere specifically with cellular pathways involved in signal transduction from the cell membrane to the nucleus. Our data demonstrate that BLV ...
Evaluation of the enzyme-linked immunosorbent assay for the rapid screening and detection of classical swine fever virus antigens in the blood of pigs.
collected from six pigs at up to fourteen days after inoculation with classical swine fever virus. All ELISA s were of the double antibody sandwich type, using monoclonal and/or polyclonal antibodies to ...
Epidemiological evaluation of a monoclonal ELISA detecting antibodies against bovine leukaemia virus in serum pools.
predictive values of an enzyme-linked immunosorbent assay (ELISA) for detecting antibodies against bovine leukaemia virus (BLV) were evaluated using a representative sample of 145 serum pools, comprising from ...
YscN, the putative energizer of the Yersinia Yop secretion machinery.
a modified YscN protein lacking box A. This mutant, impaired in Yop secretion, can be complemented in trans by a cloned yscN gene. We conclude that YscN is a component of the Yop secretion machinery using ATP. ...
Characterization of structural and non-structural proteins of hog cholera virus by means of monoclonal antibodies.
characterized by radioimmunoprecipitation assays. Using this panel, we were able to identify 4 sets of monoclonal antibodies precipitating each a different viral protein with relative molecular weight of 40, 46, ...
Diagnosis of bovine brucellosis by enzyme immunoassay of milk.
milk Predictive Value of Tests Abstract: Enzyme-immunoassays using lipopolysaccharide as antigen were developed for the detection of bovine IgG1, IgG2 or IgA Brucella antibodies (Ab) in milk. The results ...