Bacterial identification

Last updated on 13-7-2025 by Anonymous (not verified)

Test description

Description:

  • Confirmation of identification by biochemical determination. 
  • Serotyping is performed by the carrier agglutination method.
  • Re-isolation of the strain on selective medium in case the culture obtained is not pure.
  • In case of doubt, a specific in-house developed PCR is performed to identify and determine the serogroup.

Results:

Species name of the Listeria strain and serovar.

Purpose of the test

Confirmation of the identification and serotyping of isolated Listeria monocytogenes strains.

Criteria for performing this test in the context of reference activities

Confirmation of Listeria identification and serotyping of all samples isolated from patients showing symptoms of listeriosis (analysis performed in the framework of NRC Listeria surveillance)

Instructions for samples

  • Type: Isolated strains of Listeria on a transport medium or other solid medium. Exceptionally, a DNA extract of the sample may also be sent. 
  • Minimum quantity: Not applicable.

Instructions for transport

The strain is sent at room temperature and must be packed in a double protective container for safety reasons. This container must be hermetically sealed.

Unacceptable applications

Not applicable.

Turnaround time (and frequency of analysis)

  • Turnaround time: 10 working days 
  • Analysis days: Any weekday ( excluding: public holidays, extended weekends and the period between Christmas and New Year).

Reporting of test results

The analysis report is sent in the manner requested by the applicant.

 

Pathogen info

Pathogen(s): 
Listeria monocytogenes

Accreditation

Is the analysis accredited?

Materials and methods

Material(s): 
Method reference: 
SOP 12/LI/04/N

Turnaround time and time slots

Turnaround time: 
10 days

Service in charge of the analysis

Contact person(s)

Contact email

Analysis categories

Medical

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