Salmonella serotyping

Test description

Description:

• Determination of the serotype (serovar) of Salmonella enterica subsp. enterica (subspecies I) using a combination of classical glass plate agglutination according to the Kauffmann-White scheme and molecular genoserotyping (Luminex-based method).
• Performance of complementary biochemical analyses for the determination of subspecies II, IIIa, IIIb, IV, and VI and of Salmonella bongori (former subspecies V).
• Re-isolation on selective medium if the culture received by the NRC is not pure.

Results:

• Identification of the serotype (serovar) of Salmonella.

Purpose of the test

Characterisation and serotyping of Salmonella.

Criteria for performing this test in the context of reference activities

Characterisation and serotyping of all strains isolated from patients showing symptoms of salmonellosis.

Instructions for samples

• Type: The applicant sends an isolated and purified Salmonella strain in a tube transport medium. On the other hand, a culture in liquid or solid medium or exceptionally a DNA extract from a sample.
• Minimum quantity: Not applicable

Instructions for transport

Samples should be packed in a double container and stored at room temperature. The container should be hermetically sealed.

Unacceptable applications

Not applicable.

Turnaround time (and frequency of analysis)

• Turnaround time: 6 working days
• Days of analysis: Any weekday ( excluding: public holidays, extended weekends and the period between Christmas and New Year).

Reporting of test results

The analysis report is sent in the manner desired by the applicant.