Detection of norovirus by qPCR (commercial kit)

Last updated on 5-4-2024 by Anonymous (not verified)

Description of the test

Norovirus GI-GII is detected by real-time reverse-transcriptase PCR

Results: qualitative (present/absent)

Purpose of the test

Diagnosis or confirmation of diagnosis of Norovirus

Criteria for performing this test in the context of reference activities

In the context of a gastroenteritis outbreak and when the samples analysed yield a negative bacterial culture. A maximum of 5 samples are analysed per outbreak. When these result negative in PCR, additional samples are analysed.

Instructions for samples

  • Sample collection: stool (1 g), vomit (5 ml), environmental sample (swab)
  • Storage conditions: refrigerated (maximum 14 days)

Instructions for transport

Transport conditions: room temperature (maximum 18 days)

Attach an order form if the NRC is to provide the transport medium/containers

Unacceptable requests

  • Inappropriate or inadequate sample
  • Improper transport or storage conditions
  • Application form not fully completed

Turn around time (and frequency of analysis)

Turnaround time: 7 working days
Analyses can be performed any day of the working week

Reporting of test results

Norovirus diagnostic results are communicated by mail or e-mail. If upon sample receipt it is found that the sample is so damaged that further analysis is impossible, the applicant will be contacted.

Pathogen info

Pathogen(s): 
Norovirus

Accreditation

Is the analysis accredited?

Materials and methods

Material(s): 
faeces
Method reference: 
SOP 11/VM/18/N

Turnaround time and time slots

Turnaround time: 
7 days

Service in charge of the analysis

Contact email

Analysis categories

Medical

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