MIC Salmonella

Test description

Description:
Determination of antibiotic sensitivity of Salmonella or Shigella by the microdilution method. The Minimum Inhibitory Concentration (MIC) is determined for a predefined panel of antibiotics, where a suspension of a culture is incubated for 18 to 24h with series of predefined concentrations of each selected antibiotic. The following antibiotics are in the NRC panel: 

Results:
Classification of strains (resistant, intermediate, or susceptible) according to the established MIC value is done based on predefined criteria as defined by EUCAST.

Purpose of the test

Sensitivity determination of Salmonella and Shigella strains to antibiotics.

Criteria for performing this test in the context of reference activities

Susceptibility testing to antibiotics of Salmonella and Shigella strains confirmed by the NRC and isolated from patients showing symptoms of salmonellosis or shigellosis. For Salmonella, these analyses were performed on a representative proportion of strains (analysis performed as part of the NRC Salmonella-Shigella surveillance).

Instructions for samples

• Type: The applicant sends an isolated and purified Salmonella or Shigella strain in a tube of transport medium. On the other hand, a culture in liquid or solid medium.
• Minimum quantity: Not applicable.

Instructions for transport

Samples should be packed in a double container and stored at room temperature. The container should be hermetically sealed.

Unacceptable applications

Not applicable.

Turn around time (and frequency of analysis)

• Turnaround time: 15 working days
• Days of analysis: Any weekday ( except public holidays, extended weekends and the period between Christmas and New Year).

Reporting of test results

This analysis is carried out as part of surveillance. If the applicant requires a report of this analysis, it is best to contact the NRC by telephone or electronically . The analysis report will then be sent in the manner desired by the applicant.